US 11,725,252 B2
PCR detection kit for rapidly identifying Salmonella of specific serotypes
Xinan Jiao, Yangzhou (CN); Zhiming Pan, Yangzhou (CN); Dan Xiong, Yangzhou (CN); Li Song, Yangzhou (CN); Yang Jiao, Yangzhou (CN); Lin Sun, Yangzhou (CN); Xiang Chen, Yangzhou (CN); Shizhong Geng, Yangzhou (CN); Jinlin Huang, Yangzhou (CN); and Yuelan Yin, Yangzhou (CN)
Assigned to YANGZHOU UNIVERSITY, Yangzhou (CN)
Appl. No. 16/311,709
Filed by YANGZHOU UNIVERSITY, Yangzhou (CN)
PCT Filed Nov. 18, 2016, PCT No. PCT/CN2016/106398
§ 371(c)(1), (2) Date Oct. 4, 2019,
PCT Pub. No. WO2017/219597, PCT Pub. Date Dec. 28, 2017.
Claims priority of application No. 201610459944.4 (CN), filed on Jun. 22, 2016.
Prior Publication US 2020/0024647 A1, Jan. 23, 2020
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/10 (2006.01)
CPC C12Q 1/689 (2013.01) [C12Q 1/10 (2013.01)] 3 Claims
 
1. A method for detecting Salmonella enteritidis, Salmonella pullorum/gallinarum or Salmonella dublin, comprising:
(1) extracting sample genomic DNA;
(2) adding the sample genomic DNA, a positive control or a negative control into PCR tubes of a PCR reaction system respectively to obtain a corresponding sample reaction tube, positive reaction tube or negative reaction tube, wherein the PCR reaction system contains the primers comprising a forward primer having a nucleotide sequence as set forth in SEQ ID NO:1 and a reverse primer having a nucleotide sequence as set forth in SEQ ID NO:2 for detecting the tcpS gene;
(3) placing the reaction tubes on a PCR instrument, setting circulation parameters, and performing a PCR reaction; and
(4) analyzing results after the PCR reaction is completed by performing an agarose gel electrophoresis, wherein when the sample consists of at least one of Salmonella enteritidis, Salmonella pullorum/gallinarum, and Salmonella dublin, a band at 882 bp amplified by the tcpS gene forward and reverse primers is detected in the agarose gel electrophoresis;
wherein when the sample consists of none of Salmonella enteritidis, Salmonella pullorum/gallinarum, and Salmonella dublin, a band at 882 bp amplified by the tcpS gene forward and reverse primers is not detected in the agarose gel electrophoresis.