CPC C12Q 1/689 (2013.01) [C12Q 1/10 (2013.01)] | 3 Claims |
1. A method for detecting Salmonella enteritidis, Salmonella pullorum/gallinarum or Salmonella dublin, comprising:
(1) extracting sample genomic DNA;
(2) adding the sample genomic DNA, a positive control or a negative control into PCR tubes of a PCR reaction system respectively to obtain a corresponding sample reaction tube, positive reaction tube or negative reaction tube, wherein the PCR reaction system contains the primers comprising a forward primer having a nucleotide sequence as set forth in SEQ ID NO:1 and a reverse primer having a nucleotide sequence as set forth in SEQ ID NO:2 for detecting the tcpS gene;
(3) placing the reaction tubes on a PCR instrument, setting circulation parameters, and performing a PCR reaction; and
(4) analyzing results after the PCR reaction is completed by performing an agarose gel electrophoresis, wherein when the sample consists of at least one of Salmonella enteritidis, Salmonella pullorum/gallinarum, and Salmonella dublin, a band at 882 bp amplified by the tcpS gene forward and reverse primers is detected in the agarose gel electrophoresis;
wherein when the sample consists of none of Salmonella enteritidis, Salmonella pullorum/gallinarum, and Salmonella dublin, a band at 882 bp amplified by the tcpS gene forward and reverse primers is not detected in the agarose gel electrophoresis.
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