CPC C12Q 1/6874 (2013.01) [C12Q 1/6869 (2013.01); C12Q 2525/179 (2013.01); C12Q 2525/191 (2013.01)] | 16 Claims |
1. A method for detecting duplicate sequencing reads, the method comprising:
obtaining amplicons each comprising an amplified fragment of a nucleic acid with an appended adaptor, wherein each adaptor comprises an identifier site comprising a plurality of nucleotides unique to the amplified fragment;
sequencing the amplicons to generate sequence reads that include identifier and target sequences; and
identifying sequence reads with identical identifier and target sequences as duplicates.
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