US 11,725,241 B2
Compositions and methods for identification of a duplicate sequencing read
Douglas A. Amorese, Los Altos, CA (US); Jonathan Scolnick, San Francisco, CA (US); and Ben Schroeder, San Mateo, CA (US)
Assigned to Tecan Genomics, Inc., Redwood City, CA (US)
Filed by Tecan Genomics, Inc., Redwood City, CA (US)
Filed on Jul. 21, 2021, as Appl. No. 17/381,888.
Application 15/406,002 is a division of application No. 14/540,917, filed on Nov. 13, 2014, granted, now 9,546,399.
Application 17/381,888 is a continuation of application No. 16/743,604, filed on Jan. 15, 2020, granted, now 11,098,357.
Application 16/743,604 is a continuation of application No. 15/406,002, filed on Jan. 13, 2017, granted, now 10,570,448.
Claims priority of provisional application 61/903,826, filed on Nov. 13, 2013.
Prior Publication US 2021/0355537 A1, Nov. 18, 2021
Int. Cl. C12Q 1/6874 (2018.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12Q 1/6869 (2013.01); C12Q 2525/179 (2013.01); C12Q 2525/191 (2013.01)] 16 Claims
 
1. A method for detecting duplicate sequencing reads, the method comprising:
obtaining amplicons each comprising an amplified fragment of a nucleic acid with an appended adaptor, wherein each adaptor comprises an identifier site comprising a plurality of nucleotides unique to the amplified fragment;
sequencing the amplicons to generate sequence reads that include identifier and target sequences; and
identifying sequence reads with identical identifier and target sequences as duplicates.