US 11,725,239 B2
Quantitative analysis of single-cell proteins by identification and quantification separation (duet)
Long Cai, Pasadena, CA (US); Chang Ho Sohn, Seoul (KR); and Yandong Zhang, Pasadena, CA (US)
Assigned to CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
Filed by California Institute of Technology, Pasadena, CA (US)
Filed on Nov. 24, 2020, as Appl. No. 17/103,757.
Claims priority of provisional application 62/940,053, filed on Nov. 25, 2019.
Prior Publication US 2021/0180127 A1, Jun. 17, 2021
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6818 (2018.01); C12Q 1/6825 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6825 (2013.01)] 20 Claims
 
1. A method for identifying one or more macromolecules in a plurality of cells with single cell resolution, wherein each macromolecule is a nucleic acid, protein, sugar, or lipid, and wherein each macromolecule comprises a tag, the method comprising the steps of:
a. labelling, in the plurality of cells, the one or more macromolecules with one or more labels to form one or more labelled macromolecules, wherein each label comprises an oligonucleotide covalently linked to at least one moiety, and Wherein the at least one moiety is covalently or non-covalently linked to the tag on each of the one or more labelled macromolecules;
b. pooling the plurality of cells;
c. lysing the pooled plurality of cells;
d. separating each of the one or more labelled macromolecules from the lysed plurality of cells; and
e. for each of the one or more labelled macromolecules, separately:
i. identifying each macromolecule;
ii. decoding the label of each macromolecule, thereby identifying the cell that comprised the labelled macromolecule.