US 11,725,233 B2
Systems and methods for identifying and distinguishing genetic samples
Robert Charles Kain, San Diego, CA (US); and Richard Shen, Rancho Santa Fe, CA (US)
Assigned to REVERE BIOSENSORS, LLC, Worcester, MA (US)
Appl. No. 15/776,244
Filed by REVERE BIOSENSORS, LLC, Birmingham, MI (US)
PCT Filed Nov. 15, 2016, PCT No. PCT/US2016/062090
§ 371(c)(1), (2) Date May 15, 2018,
PCT Pub. No. WO2017/087416, PCT Pub. Date May 26, 2017.
Claims priority of provisional application 62/256,049, filed on Nov. 16, 2015.
Claims priority of provisional application 62/306,597, filed on Mar. 10, 2016.
Prior Publication US 2020/0087717 A1, Mar. 19, 2020
Int. Cl. C12Q 1/6837 (2018.01)
CPC C12Q 1/6837 (2013.01) [C12Q 2525/117 (2013.01); C12Q 2525/161 (2013.01); C12Q 2525/185 (2013.01); C12Q 2527/107 (2013.01); C12Q 2537/143 (2013.01); C12Q 2563/107 (2013.01)] 15 Claims
 
1. A method, comprising:
(a) providing a sample comprising nucleic acids from a plurality of subjects;
(b) contacting the nucleic acids to a biochip comprising a plurality of features each feature comprising at least a first plurality of identical probes and a second plurality of identical probes, wherein for at least a first feature, the first plurality of identical probes target a first subject-specific attribute of a first subject and the second plurality of identical probes target a second subject-specific attribute of the first subject, wherein the first plurality of identical probes and the second plurality of identical probes are encompassed in an area equal or less than an area defined by a resolving power of a detecting system;
(c) detecting using the detecting system a plurality of signals associated with binding of at least a subset of the nucleic acids to the probes of at least a subset of the plurality of features, such that i) a signal associated with said first plurality of identical probes and ii) a signal associated with said second plurality are integrated into a single resolution element; and
(d) determining an identity of at least a subset of the plurality of subjects based on the single resolution element, wherein the identity comprises a species or strain identity.