CPC C12P 19/56 (2013.01) [A23L 2/60 (2013.01); A23L 27/36 (2016.08); C07H 15/256 (2013.01); C07K 14/39 (2013.01); C07K 14/395 (2013.01); C12N 9/0036 (2013.01); C12N 9/0042 (2013.01); C12N 9/0073 (2013.01); C12N 9/0083 (2013.01); C12N 9/1051 (2013.01); C12N 9/16 (2013.01); C12N 9/2405 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12P 15/00 (2013.01); A23V 2002/00 (2013.01)] | 8 Claims |
1. A process for producing steviol and/or a steviol glycoside, comprising:
fermenting, in a suitable fermentation medium, a recombinant yeast that produces steviol and/or a steviol glycoside, and
wherein said recombinant yeast has been modified in its genome to delete, disrupt, or replace at least one gene that encodes:
(i) a phosphatase that acts on geranylgeranylpyrophosphate (GGPP) to result in the formation of geranylgeraniol (GOH);
(ii) a phosphatase that acts on farnesylpyrophosphate (FPP) to result in the formation of farnesol and nerolidol;
(iii) an exo-1,3-β glucanase;
(iv) a glycogen synthase;
(v) a transcriptional repressor of hypoxic genes (ROX1);
(vi) an NADPH oxidase;
(vii) a monocarboxylate transporter (JEN1);
(viii) a YJL064w polypeptide; or
(ix) a YPL062w polypeptide.
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