US 11,725,223 B2
Microorganisms for diterpene production
Viktor Marius Boer, Echt (NL); and Erwin Suir, Echt (NL)
Assigned to DSM IP ASSETS B.V., TE Heerlen (NL)
Filed by DSM IP ASSETS B.V., Heerlen (NL)
Filed on Mar. 30, 2020, as Appl. No. 16/834,756.
Application 16/834,756 is a division of application No. 14/893,216, granted, now 10,689,681, previously published as PCT/EP2014/061399, filed on Jun. 2, 2014.
Application 14/893,216 is a continuation of application No. 13/907,795, filed on May 31, 2013, abandoned.
Prior Publication US 2020/0283815 A1, Sep. 10, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/56 (2006.01); C12N 9/88 (2006.01); C12N 9/02 (2006.01); C12N 9/16 (2006.01); C12N 15/52 (2006.01); C12N 9/90 (2006.01); C07K 14/39 (2006.01); C12N 9/10 (2006.01); C12N 9/24 (2006.01); A23L 27/30 (2016.01); A23L 2/60 (2006.01); C07K 14/395 (2006.01); C12P 15/00 (2006.01); C07H 15/256 (2006.01)
CPC C12P 19/56 (2013.01) [A23L 2/60 (2013.01); A23L 27/36 (2016.08); C07H 15/256 (2013.01); C07K 14/39 (2013.01); C07K 14/395 (2013.01); C12N 9/0036 (2013.01); C12N 9/0042 (2013.01); C12N 9/0073 (2013.01); C12N 9/0083 (2013.01); C12N 9/1051 (2013.01); C12N 9/16 (2013.01); C12N 9/2405 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12P 15/00 (2013.01); A23V 2002/00 (2013.01)] 8 Claims
 
1. A process for producing steviol and/or a steviol glycoside, comprising:
fermenting, in a suitable fermentation medium, a recombinant yeast that produces steviol and/or a steviol glycoside, and
wherein said recombinant yeast has been modified in its genome to delete, disrupt, or replace at least one gene that encodes:
(i) a phosphatase that acts on geranylgeranylpyrophosphate (GGPP) to result in the formation of geranylgeraniol (GOH);
(ii) a phosphatase that acts on farnesylpyrophosphate (FPP) to result in the formation of farnesol and nerolidol;
(iii) an exo-1,3-β glucanase;
(iv) a glycogen synthase;
(v) a transcriptional repressor of hypoxic genes (ROX1);
(vi) an NADPH oxidase;
(vii) a monocarboxylate transporter (JEN1);
(viii) a YJL064w polypeptide; or
(ix) a YPL062w polypeptide.