CPC C12P 7/62 (2013.01) [C12N 1/20 (2013.01)] | 20 Claims |
1. A process for converting a glucose-containing hydrolyzed biomass into a fusel lactate, the process comprising:
expressing an esterase gene in a bacterial host, the bacterial host being added to or already present in the glucose-containing hydrolyzed biomass;
maintaining conditions suitable for fermenting in the hydrolyzed biomass, the fermenting producing an alcohol and a lactate; and
catalyzing through an enzyme encoded by the esterase gene, a reaction from the alcohol and lactate to form a fusel lactate in the bacterial host;
wherein the enzyme is selected from the group consisting of: ethyl-ester-synthase-1, acetylxylan-esterase-2, carbohydrate-esterase, esterase-A, truncated-esterase-A, and combinations thereof;
wherein the esterase gene is obtained from an organism selected from: Komagataella phaffii, Saccharomyces cerevisiae, Brettanomyces bruxellensis, or Pseudomonas aeruginosa.
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