CPC C12N 15/1137 (2013.01) [A61K 9/0085 (2013.01); C12N 15/113 (2013.01); C12N 2310/11 (2013.01); C12N 2310/113 (2013.01); C12N 2310/31 (2013.01); C12N 2310/315 (2013.01); C12N 2310/321 (2013.01); C12N 2310/322 (2013.01); C12N 2310/3231 (2013.01); C12N 2310/3341 (2013.01); C12N 2310/351 (2013.01); C12N 2310/3515 (2013.01); C12N 2310/3535 (2013.01); C12N 2320/32 (2013.01)] | 33 Claims |
1. A compound comprising a first oligomeric compound and a second oligomeric compound, wherein the first oligomeric compound comprises a first modified oligonucleotide consisting of 14-20 linked nucleosides and has a nucleobase sequence complementary to the nucleobase sequence of the second oligomeric compound and to a nucleic acid target; and
the second oligomeric compound comprises a second modified oligonucleotide consisting of 6-12 linked nucleosides;
wherein the first modified oligonucleotide has a sugar motif comprising:
a 5′-region consisting of 1-5 linked 5′-nucleosides,
a central region consisting of 6-10 linked central region nucleosides, and
a 3′-region consisting of 1-5 linked 3′-nucleosides; wherein
each of the 5′-region nucleosides and each of the 3′-region comprises a modified sugar moiety and each of the central region nucleosides comprises an unmodified DNA sugar moiety; and
wherein the second modified oligonucleotide has a sugar motif comprising:
a 5′-region consisting of 2-4 linked 5′-nucleosides,
a central region consisting of 2-4 linked central region nucleosides, and
a 3′-region consisting of 2-4 linked 3′-nucleosides; wherein
each of the 5′-region nucleosides and each of the 3′-region comprises a modified sugar moiety and each of the central region nucleosides comprises an unmodified DNA sugar moiety.
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