US 11,725,197 B2
Lipase variants and polynucleotides encoding same
Allan Svendsen, Hoersholm (DK); Sune Fang Christensen, Bagsvaerd (DK); Lars Lehman Hylling Christensen, Holte (DK); Vikram Kjoller Bhatia, Charlottelund (DK); Jesper Vind, Vaerlose (DK); and Vibeke Skovsgaard Nielsen, Nordhavn (DK)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Appl. No. 16/765,247
Filed by Novozymes A/S, Bagsvaerd (DK)
PCT Filed Dec. 3, 2018, PCT No. PCT/EP2018/083261
§ 371(c)(1), (2) Date May 19, 2020,
PCT Pub. No. WO2019/110462, PCT Pub. Date Jun. 13, 2019.
Claims priority of application No. 17205251 (EP), filed on Dec. 4, 2017.
Prior Publication US 2020/0347368 A1, Nov. 5, 2020
Int. Cl. C12N 9/20 (2006.01); C11D 1/83 (2006.01); C11D 3/386 (2006.01); C11D 11/00 (2006.01); C11D 1/14 (2006.01); C11D 1/22 (2006.01); C11D 1/72 (2006.01)
CPC C12N 9/20 (2013.01) [C11D 1/83 (2013.01); C11D 3/38627 (2013.01); C11D 11/0017 (2013.01); C12Y 301/01003 (2013.01); C11D 1/146 (2013.01); C11D 1/22 (2013.01); C11D 1/72 (2013.01)] 11 Claims
 
1. A variant of a parent lipase, wherein said variant has:
a) at least 90% but less than 100% sequence identity with SEQ ID NO: 2;
b) lipase activity;
c) substitutions corresponding to G91A, N92D, and K98I in the region located from position G82 to position 1100 of SEQ ID NO: 2; and
d) substitutions corresponding to T231R and N233R of SEQ ID NO: 2.