CPC C12N 9/0006 (2013.01) [C12N 15/70 (2013.01); C12Q 1/32 (2013.01)] | 20 Claims |
1. A modified glucose dehydrogenase selected from the group consisting of consisting of
an amino acid sequence represented by SEQ ID NO: 2 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 3 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 24 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 25 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 26 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 27 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2; and
an amino acid sequence represented by SEQ ID NO: 28 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
wherein the modified glucose dehydrogenase has, after a treatment in 0.1M phosphate buffer (pH 6.0) at 45° C. for 30 minutes, a residual activity greater than that of a wild-type enzyme.
|