US 11,725,193 B2
Modified glucose dehydrogenase
Michinari Honda, Hiroshima (JP); Hirokazu Sanada, Hiroshima (JP); Ryo Takenaka, Hiroshima (JP); Takafumi Takumi, Hiroshima (JP); and Takahiro Fujii, Hiroshima (JP)
Assigned to IKEDA FOOD RESEARCH CO., LTD., Hiroshima (JP)
Appl. No. 16/619,637
Filed by IKEDA FOOD RESEARCH CO., LTD., Hiroshima (JP)
PCT Filed May 25, 2018, PCT No. PCT/JP2018/020118
§ 371(c)(1), (2) Date Apr. 9, 2020,
PCT Pub. No. WO2018/230304, PCT Pub. Date Dec. 20, 2018.
Claims priority of application No. 2017-116585 (JP), filed on Jun. 14, 2017.
Prior Publication US 2022/0098557 A1, Mar. 31, 2022
Int. Cl. C12N 9/04 (2006.01); C12N 15/70 (2006.01); C12Q 1/32 (2006.01)
CPC C12N 9/0006 (2013.01) [C12N 15/70 (2013.01); C12Q 1/32 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A modified glucose dehydrogenase selected from the group consisting of consisting of
an amino acid sequence represented by SEQ ID NO: 2 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 3 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 24 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 25 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 26 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
an amino acid sequence represented by SEQ ID NO: 27 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2; and
an amino acid sequence represented by SEQ ID NO: 28 and at least one amino acid substitution at a position corresponding to S71, G72, Q169, S188, G210, A221, S269, N346, S427, S429, F521 or V567 of SEQ ID NO: 2;
wherein the modified glucose dehydrogenase has, after a treatment in 0.1M phosphate buffer (pH 6.0) at 45° C. for 30 minutes, a residual activity greater than that of a wild-type enzyme.