	US 11,725,192 B2
	Separation and quantification of empty and full viral capsid particles
Santoshkumar Khatwani, Raleigh, NC (US); and Zhu Pirot, Redwood City, CA (US)
Assigned to SANGAMO THERAPEUTICS, INC., Brisbane, CA (US)
Filed by Sangamo Therapeutics, Inc., Brisbane, CA (US)
Filed on Jul. 12, 2020, as Appl. No. 16/926,732.
Claims priority of provisional application 62/873,619, filed on Jul. 12, 2019.
Prior Publication US 2021/0009964 A1, Jan. 14, 2021
Int. Cl. C12N 7/00 (2006.01); B01D 15/00 (2006.01); B01D 15/16 (2006.01); B01D 15/36 (2006.01); B01D 15/42 (2006.01)

CPC C12N 7/00 (2013.01) [B01D 15/166 (2013.01); B01D 15/363 (2013.01); B01D 15/424 (2013.01); C12N 2750/14151 (2013.01)]

24 Claims

1. A method of separating empty and full capsids in a viral preparation, the method comprising running the viral preparation and a mobile phase through an anion exchange column, wherein the mobile phase is run under conditions comprising a discontinuous elution gradient and at least one isocratic hold incorporated into the gradient.