US 11,725,174 B2
Cell culture apparatus and cell culture method
Shinichi Nakai, Kanagawa (JP); Yoichi Nagai, Kanagawa (JP); Nobuyuki Haraguchi, Kanagawa (JP); and Shigehisa Sugiyama, Kanagawa (JP)
Assigned to FUJIFILM CORPORATION, Tokyo (JP)
Filed by FUJIFILM CORPORATION, Tokyo (JP)
Filed on Jul. 2, 2019, as Appl. No. 16/460,983.
Application 16/460,983 is a continuation of application No. PCT/JP2018/008175, filed on Mar. 2, 2018.
Claims priority of application No. 2017-040956 (JP), filed on Mar. 3, 2017.
Prior Publication US 2019/0322975 A1, Oct. 24, 2019
Int. Cl. B01D 61/14 (2006.01); B01D 61/18 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); B01D 61/58 (2006.01); C12N 5/00 (2006.01)
CPC C12M 29/04 (2013.01) [B01D 61/145 (2013.01); B01D 61/146 (2022.08); B01D 61/147 (2013.01); B01D 61/18 (2013.01); B01D 61/58 (2013.01); C12M 29/10 (2013.01); C12M 29/18 (2013.01); C12M 33/14 (2013.01); C12M 37/02 (2013.01); C12M 39/00 (2013.01); C12M 47/02 (2013.01); C12N 5/0081 (2013.01); B01D 2311/25 (2013.01); B01D 2311/2688 (2013.01); B01D 2315/08 (2013.01); B01D 2315/10 (2013.01); B01D 2317/025 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A cell culture apparatus comprising:
a culture vessel that stores a cell suspension containing cells;
a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel;
a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel;
a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane;
a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and
a recovery flow path that recovers components blocked by the second filter membrane,
wherein an average hole diameter of the first filter membrane is from 0.05 μm to 20 μm, and
the second filter membrane is an ultrafiltration membrane having a molecular weight cut-off of 3 kDa or more.