US 11,725,062 B2
Method for improving affinity of antibody for antigen and use thereof
Shingo Maeta, Kobe (JP); Atsushi Fukunaga, Kobe (JP); and Reema Bajaj, Kobe (JP)
Assigned to SYSMEX CORPORATION, Kobe (JP)
Filed by SYSMEX CORPORATION, Kobe (JP)
Filed on Jan. 21, 2021, as Appl. No. 17/154,297.
Claims priority of application No. 2020-010411 (JP), filed on Jan. 24, 2020; application No. 2020-010413 (JP), filed on Jan. 24, 2020; application No. 2020-010414 (JP), filed on Jan. 24, 2020; application No. 2020-010416 (JP), filed on Jan. 24, 2020; application No. 2020-010417 (JP), filed on Jan. 24, 2020; application No. 2020-010418 (JP), filed on Jan. 24, 2020; application No. 2020-010419 (JP), filed on Jan. 24, 2020; application No. 2020-010420 (JP), filed on Jan. 24, 2020; application No. 2020-093384 (JP), filed on May 28, 2020; application No. 2020-093389 (JP), filed on May 28, 2020; application No. 2020-093392 (JP), filed on May 28, 2020; application No. 2020-093399 (JP), filed on May 28, 2020; application No. 2020-093405 (JP), filed on May 28, 2020; application No. 2020-093411 (JP), filed on May 28, 2020; application No. 2020-093421 (JP), filed on May 28, 2020; and application No. 2020-093424 (JP), filed on May 28, 2020.
Prior Publication US 2021/0230298 A1, Jul. 29, 2021
Int. Cl. C07K 16/00 (2006.01); C07K 16/32 (2006.01)
CPC C07K 16/32 (2013.01) [C07K 2317/51 (2013.01); C07K 2317/54 (2013.01); C07K 2317/55 (2013.01); C07K 2317/567 (2013.01); C07K 2317/569 (2013.01); C07K 2317/622 (2013.01)] 12 Claims
 
1. A method for improving affinity of an antibody or an antibody fragment for an antigen, comprising improving affinity of the antibody or the antibody fragment by changing 3rd, 5th and 9th amino acid residues defined by Kabat method of a light chain of the antibody or the antibody fragment to charged amino acid residues.