	US 11,725,059 B2
	Antibodies targeting B-cell maturation antigen and methods of use
Renier J. Brentjens, New York, NY (US); Eric L. Smith, New York, NY (US); and Cheng Liu, Emeryville, CA (US)
Assigned to MEMORIAL SLOAN KETTERING CANCER CENTER, New York, NY (US); and EUREKA THERAPEUTICS, INC., Emeryville, CA (US)
Filed by MEMORIAL SLOAN KETTERING CANCER CENTER, New York, NY (US); and EUREKA THERAPEUTICS, INC., Emeryville, CA (US)
Filed on Feb. 11, 2021, as Appl. No. 17/173,716.
Application 17/173,716 is a continuation of application No. 16/732,089, filed on Dec. 31, 2019, granted, now 10,947,314.
Application 16/732,089 is a continuation of application No. 15/613,986, filed on Jun. 5, 2017, granted, now 10,562,972, issued on Feb. 18, 2020.
Application 15/613,986 is a continuation of application No. PCT/US2015/064119, filed on Dec. 4, 2015.
Claims priority of provisional application 62/088,246, filed on Dec. 5, 2014.
Prior Publication US 2022/0315660 A1, Oct. 6, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/395 (2006.01); C07K 16/28 (2006.01); C07K 14/705 (2006.01); A61K 47/68 (2017.01); A61P 35/00 (2006.01); G01N 33/68 (2006.01)

CPC C07K 16/2878 (2013.01) [A61K 47/6849 (2017.08); A61P 35/00 (2018.01); C07K 14/70575 (2013.01); C07K 16/2896 (2013.01); G01N 33/6863 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/34 (2013.01); C07K 2317/622 (2013.01); C07K 2317/92 (2013.01); C07K 2319/21 (2013.01); C07K 2319/30 (2013.01); C07K 2319/42 (2013.01); G01N 2333/70578 (2013.01)]

40 Claims

1. A method of treating a tumor associated with B-cell maturation antigen (BCMA) in a subject, comprising administering to the subject an effective amount of an anti-BCMA single-chain variable fragment (scFv) comprising a heavy chain variable region and a light chain variable region, wherein:

(a) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 53, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 54;

(b) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 65, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 66;

(c) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 21, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 22;

(d) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 1, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 2;

(e) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 5, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 6;

(f) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 9, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 10;

(g) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 13, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 14;

(h) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 17, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 18;

(i) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 25, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 26;

(j) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 29, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 30;

(k) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 33, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 34;

(l) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 37, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 38;

(m) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 41, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 42;

(n) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 45, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 46;

(o) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 49, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 50;

(p) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 57, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 58; or

(q) the heavy chain variable region comprises the CDR1, CDR2, and CDR3 of the heavy chain variable sequence set forth in SEQ ID NO: 61, and the light chain variable region comprises the CDR1, CDR2, and CDR3 of the light chain variable region sequence set forth in SEQ ID NO: 62.