US 11,725,042 B2
Methods and devices for complexing target entities
Adil Kassam, Vancouver (CA); and Eric Jervis, Vancouver (CA)
Assigned to STEMCELL TECHNOLOGIES CANADA INC., Vancouver (CA)
Filed by STEMCELL Technologies Canada Inc., Vancouver (CA)
Filed on Jun. 26, 2020, as Appl. No. 16/912,882.
Claims priority of provisional application 62/867,434, filed on Jun. 27, 2019.
Prior Publication US 2020/0407417 A1, Dec. 31, 2020
Int. Cl. C07K 14/705 (2006.01); C07K 16/28 (2006.01); G01N 33/48 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/541 (2006.01); G01N 33/543 (2006.01); G01N 33/566 (2006.01); G01N 33/577 (2006.01); A61K 47/69 (2017.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); G01N 33/537 (2006.01)
CPC C07K 14/70596 (2013.01) [A61K 47/6913 (2017.08); C07K 16/2896 (2013.01); C12M 21/00 (2013.01); C12M 25/14 (2013.01); C12M 41/36 (2013.01); G01N 33/48 (2013.01); G01N 33/50 (2013.01); G01N 33/53 (2013.01); G01N 33/537 (2013.01); G01N 33/541 (2013.01); G01N 33/54346 (2013.01); G01N 33/566 (2013.01); G01N 33/577 (2013.01); C07K 2319/03 (2013.01); G01N 2333/705 (2013.01); G01N 2333/70596 (2013.01)] 12 Claims
 
1. A method of recovering a population of extracellular vesicles from a biological sample comprising extracellular vesicles and contaminants, the method comprising:
a) removing contaminants from the sample, wherein said contaminants are larger or more dense than the extracellular vesicles;
b) contacting the sample of step a) with a plurality of binding compositions, each binding composition having first and/or second moieties linked by third and fourth moieties, wherein the first and/or second moieties bind a recognition motif of extracellular vesicles under conditions to allow aggregation of the extracellular vesicles and the plurality of binding compositions to form a complexed population of extracellular vesicles, the complexed population of extracellular vesicles having an increased volume and/or higher density in comparison to the extracellular vesicles in individual form and wherein the complexed population of extracellular vesicles is formed without reliance on physical components other than the plurality of binding compositions; and
c) recovering the complexed population of extracellular vesicles, wherein the recognition motif is one or more of CD9, CD63, and CD81 and wherein the first, second, third, and fourth moieties are antibodies or fragments thereof.