CPC A61K 39/12 (2013.01) [A61K 38/20 (2013.01); A61K 38/208 (2013.01); A61K 38/2086 (2013.01); A61N 1/327 (2013.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); A61K 2039/53 (2013.01); A61K 2039/545 (2013.01); A61K 2039/57 (2013.01); A61K 2039/572 (2013.01); A61K 2039/70 (2013.01); C07K 2319/02 (2013.01); C12N 2760/14122 (2013.01); C12N 2760/14134 (2013.01); C12N 2760/14171 (2013.01); C12N 2760/14222 (2013.01); C12N 2760/14234 (2013.01); C12N 2760/14271 (2013.01)] | 11 Claims |
1. A composition comprising one or more nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
a) a nucleic acid sequence that encodes a first consensus Marburg marburgvirus envelope glycoprotein immunogen, wherein the amino acid sequence of the first consensus Marburg marburgvirus envelope glycoprotein immunogen is selected from the group consisting of: SEQ ID NO:4 (MARV CON1), a fragment of SEQ ID NO:4 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:4, SEQ ID NO:4 (MARV CON1) linked to an IgE signal peptide, and a fragment of SEQ ID NO:4 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:4 linked to an IgE signal peptide;
b) a nucleic acid sequence that encodes a second consensus Marburg marburgvirus envelope glycoprotein immunogen, wherein the amino acid sequence of the second consensus Marburg marburgvirus envelope glycoprotein immunogen is selected from the group consisting of: SEQ ID NO:5 (MARV CON2), a fragment of SEQ ID NO:5 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:5, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:5, a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:5 over 600 or more consecutive amino acids, SEQ ID NO:5 (MARV CON2) linked to an IgE signal peptide, a fragment of SEQ ID NO:5 that comprises a sequence having 100% identity over 600 or more consecutive amino acids linked to an IgE signal peptide, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:5 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:5 that comprises 600 or more consecutive amino acids of SEQ ID NO:5 linked to an IgE signal peptide; and
c) a nucleic acid sequence that encodes a second consensus Marburg marburgvirus envelope glycoprotein immunogen, wherein the amino acid sequence of the second consensus Marburg marburgvirus envelope glycoprotein immunogen is selected from the group consisting of: SEQ ID NO:6 (MARV CON3), a fragment of SEQ ID NO:6 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:6, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:6, a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:6 over 600 or more consecutive amino acids, SEQ ID NO:6 (MARV CON3) linked to an IgE signal peptide, a fragment of SEQ ID NO:6 that comprises a sequence having 100% identity over 600 or more consecutive amino acids linked to an IgE signal peptide, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:6 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:6 that comprises 600 or more consecutive amino acids of SEQ ID NO:6 linked to an IgE signal peptide.
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