Microorganisms programmed to produce immune modulators and anti-cancer therapeutics in tumor cells
Dean Falb, Sherborn, MA (US); Jonathan W. Kotula, Somerville, MA (US); Vincent M. Isabella, Cambridge, MA (US); Paul F. Miller, Salem, CT (US); Suman Machinani, Cambridge, MA (US); Saurabh Saha, Wellesley Hills, MA (US); Adam B. Fisher, Cambridge, MA (US); Yves Millet, Newton, MA (US); and Ning Li, Winchester, MA (US)
Assigned to Synlogic Operating Company, Inc., Cambridge, MA (US)
Appl. No. 16/69,220
Filed by Synlogic Operating Company, Inc., Cambridge, MA (US)
1. A genetically engineered non-pathogenic microorganism for intratumoral administration comprising a gene sequence for producing kynureninase, wherein the gene sequence is operably linked to an inducible promoter, and wherein the bacterium further comprises an aroP, tnaB, or mtr gene sequence encoding a transporter for importing kynurenine into the bacterium; and wherein the bacterium is an auxotroph comprising a deletion in a thyA, dapD, or dapA gene.