US 10,376,599 B2
Nanoplatforms for arginase, indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase detection by posttranslational modification
Stefan H. Bossmann, Manhattan, KS (US); Deryl L. Troyer, Manhattan, KS (US); Aruni P. Malalasekera, Manhattan, KS (US); Hongwang Wang, Manhattan, KS (US); Sebastian O. Wendel, Manhattan, KS (US); and Gaohong Zhu, Yunnan (CN)
Assigned to Kansas State University Research Foundation, Manhattan, KS (US)
Appl. No. 15/559,543
Filed by Kansas State University Research Foundation, Manhattan, KS (US)
PCT Filed Mar. 18, 2016, PCT No. PCT/US2016/023188
§ 371(c)(1), (2) Date Sep. 19, 2017,
PCT Pub. No. WO2016/149637, PCT Pub. Date Sep. 22, 2016.
Claims priority of provisional application 62/135,366, filed on Mar. 19, 2015.
Prior Publication US 2018/0099057 A1, Apr. 12, 2018
Int. Cl. A61K 38/00 (2006.01); A61K 49/00 (2006.01); G01N 33/573 (2006.01); C12Q 1/26 (2006.01); C12Q 1/34 (2006.01)
CPC A61K 49/0093 (2013.01) [A61K 49/0032 (2013.01); A61K 49/0036 (2013.01); A61K 49/0056 (2013.01); C12Q 1/26 (2013.01); C12Q 1/34 (2013.01); C12Y 113/11011 (2013.01); C12Y 113/11052 (2013.01); C12Y 305/03001 (2013.01); G01N 33/573 (2013.01)] 21 Claims
 
1. A nanoplatform assembly for detecting the presence of an active enzyme in a sample, wherein said enzyme is arginase, said nanoplatform comprising:
a central carrier particle;
a first particle;
a second particle; and
an oligopeptide linkage connecting said central carrier particle and said second particle,
wherein said first particle is directly attached to said central carrier particle, and wherein said oligopeptide linkage comprises a substrate sequence for said enzyme, wherein said substrate sequence comprises a sequence according to (I) or (II):
(X)n(R)m(X)l (SEQ ID NO:1),  (I)
where:
each R: arginine;
each X: any amino acid;
m: 3-10;
n: 1-6; and
l: 1-6; or
(X)n(RJ)m(X)l (SEQ ID NO:2),  (II)
where:
each R: arginine;
each X: any amino acid;
each J: E, A, L, M, K, R, Q, or H;
m: 2-20;
n: 1-6; and
l: 1-6.