US 10,376,571 B2
Compositions and methods of enhancing immune responses to enteric pathogens
Lisa Bielke, Fayetteville, AR (US); Sherryll Layton, Rogers, AR (US); Billy Hargis, Fayetteville, AR (US); Neil R. Pumford, Bentonville, AR (US); Olivia B. Faulkner, Farmington, AR (US); Luc Berghman, College Station, TX (US); and Daad Abi-Ghanem, Tigard, OR (US)
Assigned to THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS, Little Rock, AR (US); and THE TEXAS A&M UNIVERSITY SYSTEM, College Station, TX (US)
Appl. No. 14/776,986
Filed by THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS, Little Rock, AR (US); and The Texas A&M University System, College Station, TX (US)
PCT Filed Mar. 14, 2014, PCT No. PCT/US2014/027416
§ 371(c)(1), (2) Date Sep. 15, 2015,
PCT Pub. No. WO2014/152508, PCT Pub. Date Sep. 25, 2014.
Claims priority of provisional application 61/790,301, filed on Mar. 15, 2013.
Prior Publication US 2016/0038581 A1, Feb. 11, 2016
Int. Cl. C07H 21/02 (2006.01); A61K 49/00 (2006.01); A61K 39/00 (2006.01); A61K 39/112 (2006.01); A61K 39/108 (2006.01); C07K 16/12 (2006.01); A61K 39/02 (2006.01); C12N 15/85 (2006.01)
CPC A61K 39/0275 (2013.01) [A61K 39/0258 (2013.01); A61K 39/105 (2013.01); A61K 39/107 (2013.01); C07K 16/1232 (2013.01); C12N 15/85 (2013.01); A61K 2039/523 (2013.01); A61K 2039/53 (2013.01); C07K 2317/34 (2013.01); Y02A 50/474 (2018.01); Y02A 50/482 (2018.01)] 21 Claims
 
1. A vaccine vector comprising a first polynucleotide sequence encoding a PAL polypeptide consisting of a polypeptide selected from the group consisting of SEQ ID NO: 1, an amino acid sequence having 90% or more sequence identity to SEQ ID NO: 1 and an immunogenic fragment thereof at least six amino acids long and a second polynucleotide sequence encoding an HMGB1 polypeptide, wherein the PAL polypeptide and the HMGB1 polypeptide are expressed on the surface of the vaccine vector, and wherein the HMGB1 polypeptide comprises a polypeptide selected from the group consisting of at least one of SEQ ID NOs: 15-23.