US 10,376,563 B1
Methods for systemically delivering polypeptides and microorganisms therefor
Jan Peter Van Pijkeren, Madison, WI (US); Alan Attie, Madison, WI (US); Mark Keller, McFarland, WI (US); and Jee-Hwan Oh, Madison, WI (US)
Assigned to WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US)
Filed by WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US)
Filed on Feb. 13, 2017, as Appl. No. 15/431,373.
Claims priority of provisional application 62/294,578, filed on Feb. 12, 2016.
Int. Cl. C12N 15/74 (2006.01); A61K 35/74 (2015.01); A61K 38/00 (2006.01); A61K 39/09 (2006.01); A61K 38/20 (2006.01); A61K 35/747 (2015.01); A61K 38/02 (2006.01); A61K 9/00 (2006.01); C07K 14/54 (2006.01); A61K 35/00 (2006.01)
CPC A61K 38/20 (2013.01) [A61K 9/0053 (2013.01); A61K 35/747 (2013.01); A61K 38/02 (2013.01); C07K 14/54 (2013.01); A61K 2035/11 (2013.01)] 11 Claims
 
1. A method of systemically introducing a polypeptide into a bloodstream of a subject, the method comprising administering into the gastrointestinal tract of the subject a bacterium that produces and releases the polypeptide, wherein the bacterium comprises a recombinant gene configured to express the polypeptide, wherein the bacterium is administered in an amount effective to introduce the polypeptide in the bloodstream of the subject in a detectable amount without the bacterium being introduced in the bloodstream of the subject in a detectable amount, wherein the bacterium is Lactobacillus reuteri.