US 10,376,552 B2
Memory improving composition comprising arillus longan, semen nelumbinis, poria and semen juglandis
Yong Zhou, Jiang Men (CN); Xiaolei Guo, Jiang Men (CN); and Chung Wah Ma, Jiang Men (CN)
Assigned to INFINITUS (CHINA) COMPANY LTD., Jiang Men (CN)
Filed by INFINITUS (CHINA) COMPANY LTD., Jiang Men (CN)
Filed on Aug. 31, 2016, as Appl. No. 15/252,528.
Claims priority of application No. 2015 1 0573446 (CN), filed on Sep. 10, 2015.
Prior Publication US 2017/0071996 A1, Mar. 16, 2017
Int. Cl. A61K 36/52 (2006.01); A61K 31/185 (2006.01); A61K 31/685 (2006.01); A61K 36/076 (2006.01); A61K 36/185 (2006.01); A61K 36/62 (2006.01); A23L 33/105 (2016.01); A61K 36/77 (2006.01); A61K 38/01 (2006.01)
CPC A61K 36/52 (2013.01) [A23L 33/105 (2016.08); A61K 31/185 (2013.01); A61K 31/685 (2013.01); A61K 36/076 (2013.01); A61K 36/185 (2013.01); A61K 36/62 (2013.01); A61K 36/77 (2013.01); A61K 38/011 (2013.01); A23V 2002/00 (2013.01); A61K 2236/15 (2013.01); A61K 2236/19 (2013.01); A61K 2236/331 (2013.01); A61K 2236/53 (2013.01)] 5 Claims
 
1. A memory improving composition, which is made from materials consisting of 200-800 parts of Arillus Longan, 200-800 parts of Semen Nelumbinis, 200-800 parts of Poria, 10-150 parts of enzymatic extract of Semen Juglandis, 10-150 parts of phosphatidylserine, and 3-50 parts of taurine, wherein the enzymatic extract of Semen Juglandis is a hydrolysate of Semen Juglandis dregs after hydrolyzation with alkaline endoprotease Alcalase™ (Bacillus protease preparation) 2.4L and complex protease Protamex™ (Bacillus protease preparation);
wherein the enzymatic extract of Semen Juglandis is prepared by the following method:
(a) grinding Semen Juglandis dregs in water to obtain a slurry of Semen Juglandis dregs, adding alkali to adjust the pH value to 8.0 to 9.0, centrifuging, removing impurities in the supernatant enzymatically, adding acid to cause precipitation, washing the precipitate to obtain a walnut protein;
(b) hydrolyzing the walnut protein to a hydrolysis degree of 8-12% with alkaline endoprotease Alcalase™ 2.4L and complex protease Protamex™, and collecting the supernatant as enzymatic hydrolysate of walnut protein after enzyme deactivation and centrifugation;
(c) enriching enzymatic extract of Semen Juglandis by separating the enzymatic hydrolysate of walnut protein through a filter cloth and ultrafiltration membrane.