US 10,376,148 B2
System and method for laser imaging and ablation of cancer cells using fluorescence
Fred Wood, Medford, NY (US); Dmitry Yavid, Stony Brook, NY (US); Joe Zott, Menlo Park, CA (US); and Ron Goldman, Cold Spring Harbor, NY (US)
Assigned to AccuVein, Inc., Cold Spring Harbor, NY (US)
Filed by Fred Wood, Medford, NY (US); Dmitry Yavid, Stony Brook, NY (US); Joe Zott, Menlo Park, CA (US); and Ron Goldman, Cold Spring Harbor, NY (US)
Filed on Dec. 5, 2013, as Appl. No. 14/97,337.
Claims priority of provisional application 61/733,535, filed on Dec. 5, 2012.
Claims priority of provisional application 61/830,225, filed on Jun. 3, 2013.
Prior Publication US 2014/0187879 A1, Jul. 3, 2014
Int. Cl. A61B 5/00 (2006.01); A61B 18/20 (2006.01); A61B 18/00 (2006.01)
CPC A61B 5/0071 (2013.01) [A61B 5/0077 (2013.01); A61B 18/20 (2013.01); A61B 5/742 (2013.01); A61B 2018/00577 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for three-dimensional imaging of cancer cells of a target surgical area, and for projecting the imaged cancer cells directly onto the target surgical area, said method comprising:
introducing, fluorophores having affinity for targeted cancer cells into biologic tissues of the target surgical area;
emitting a beam of light at a first infrared wavelength from a first laser, a beam of light at a red wavelength from a second laser, a beam of light at a blue wavelength from a third laser, and a beam of light at a green wavelength from a fourth laser, within each of a plurality of alternate imaging frames;
co-axially aligning the beams of infrared, red, blue, and green light using a means for aligning;
scanning the co-axially aligned beam of light, using a scanner, in a pattern, and across the target surgical area, exciting the fluorophores and causing emitting of fluorescent excitation light at a second infrared wavelength during the alternate imaging frames, and causing illumination of the target surgical area with white light during the alternate imaging frames;
converting each image of the fluorescent excitation light of the fluorophores for each of the alternate imaging frames into an analog signal by a light detector;
converting the analog signal of each image into a digital image by an image processor, and successively storing each in a memory;
successively outputting each digital image of the stored image frames to one of said red, green, or blue lasers as an analog signal by the processing unit, and projecting by said one of said red, green, or blue lasers of each image onto the target surgical area during respective display frames succeeding the alternate imaging frames, using said analog signal.