US 10,376,147 B2
System and method for multi-color laser imaging and ablation of cancer cells using fluorescence
Fred Wood, Medford, NY (US); Dmitry Yavid, Stony Brook, NY (US); Joe Zott, Menlo Park, CA (US); and Ron Goldman, Cold Spring Harbor, NY (US)
Assigned to AccuVeiw, Inc., Cold Spring Harbor, NY (US)
Filed by Fred Wood, Medford, NY (US); Dmitry Yavid, Stony Brook, NY (US); Joe Zott, Menlo Park, CA (US); and Ron Goldman, Cold Spring Harbor, NY (US)
Filed on Dec. 5, 2013, as Appl. No. 14/97,323.
Claims priority of provisional application 61/733,535, filed on Dec. 5, 2012.
Claims priority of provisional application 61/830,225, filed on Jun. 3, 2013.
Prior Publication US 2014/0187967 A1, Jul. 3, 2014
Int. Cl. A61B 5/00 (2006.01); A61B 18/20 (2006.01); A61B 18/00 (2006.01)
CPC A61B 5/0071 (2013.01) [A61B 5/0077 (2013.01); A61B 18/20 (2013.01); A61B 5/742 (2013.01); A61B 2018/00577 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for three-dimensional imaging of cancer of a target surgical area comprising:
introducing fluorophores having, affinity for targeted cancer cells into biologic tissues of internal body structures of the target surgical area;
emitting light from a first laser at an infrared wavelength selected to cause excitation of said fluorophores;
scanning said emitted light at said infrared wavelength, using a means for scanning, in a two dimensional pattern, spanning across the target surgical area;
using a lens for receiving and focusing, onto a photodetector, a fluorescent excitation wavelength of light emitted by said excited fluorophores;
converting into an analog signal, by said photodetector, of an image of said fluorophores formed by said focused excitation wavelength of light;
transmitting said analog signal output from said photodetector to a processing unit;
selectively emitting light from a second laser at one or more visible wavelengths;
co-axially aligning said selectively emitted light of said second laser with said emitted light of said first laser, using a means for aligning, for scanning of said selectively emitted visible light in said pattern, using said means for scanning;
using, by said processing unit, of said analog signal, for causing said selectively emitting of said light by said second laser to be at a first visible wavelength, being a desired visible color, and for causing of said selective emitting of said first visible wavelength of light to only occur when directed at a position in said scan corresponding to said received excitation wavelength of light from said fluorophores, to create a visible three-dimensional projection on the target surgical area of the targeted cancer cells;
selectively emitting light, by a third laser, at a selective wavelength for causing ablation of the targeted cancer cells;
co-axially aligning said selectively emitted light of said ablation laser with said emitted light of said first laser, using, said means for aligning, for selectively scanning of said selective wavelength of light in said pattern, for three-dimensional projecting onto the target surgical area; and
causing, by said processing unit, of said selectively emitting of said light at said selective wavelength by said third laser for only occurring when directed at a position in said scan corresponding to said received excitation wavelength of light from said fluorophores, for ablating the targeted cancer cells.