US 11,719,922 B2
Single plane illumination microscope
Thomas Kalkbrenner, Jena (DE); Ralf Netz, Jena (DE); Helmut Lippert, Jena (DE); and Joerg Siebenmorgen, Jena (DE)
Assigned to CARL ZEISS MICROSCOPY GMBH, Jena (DE)
Filed by Carl Zeiss Microscopy GmbH, Jena (DE)
Filed on Apr. 14, 2021, as Appl. No. 17/230,613.
Application 17/230,613 is a continuation of application No. 16/342,387, granted, now 10,983,322, previously published as PCT/EP2017/076824, filed on Oct. 20, 2017.
Claims priority of application No. 10 2016 120 683.0 (DE), filed on Oct. 28, 2016.
Prior Publication US 2021/0231936 A1, Jul. 29, 2021
Int. Cl. G02B 21/00 (2006.01); G02B 21/32 (2006.01)
CPC G02B 21/0032 (2013.01) [G02B 21/006 (2013.01); G02B 21/32 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A light sheet microscope, comprising
means for generating a light sheet,
an illumination optical unit for illuminating a sample which is located on a sample carrier in a medium, said sample carrier being oriented parallel to a planar reference surface, via an illumination beam path with said light sheet, the latter having a light sheet plane that encloses an illumination angle that differs from zero with the normal of the reference surface,
a detection optical unit having a detection beam path with an optical axis, which encloses a detection angle that differs from zero with the normal of the reference surface in the region of the sample carrier,
a separation layer system with at least one layer that is made of a specified material with a specified thickness and separates the medium from the illumination and detection optical units, wherein the separation layer system is in contact, at least in the region that is accessible for illumination and detection, with the medium by way of a base surface that is parallel to the reference surface,
at least one correction element in the illumination and/or in the detection beam path for reducing such aberrations that occur due to the oblique passage of light that is to be detected or of light for illuminating the sample through interfaces of the separation layer system,
means for optical manipulation of the sample by manipulation light by causing manipulation light intensity to impinge on the sample in substantially point-shaped areas in a specified volume which at least temporarily comprises the light sheet plane, via at least one manipulation beam path,
means for sequential switching between an illumination of the sample by manipulation light and an illumination of the sample with the light sheet.