US 11,719,712 B2
Automated sample deposition and staining systems and associated methods
Hariharan Subramanian, Evanston, IL (US); Michael Verleye, Evanston, IL (US); Micah Litow, Evanston, IL (US); Justin R. Derbas, Evanston, IL (US); Sergey Rozhok, Evanston, IL (US); John Hart, Evanston, IL (US); Chester Henderson, Box Hill (AU); Rebecca Bartel, Box Hill (AU); Nathan Ray, Box Hill (AU); and Anthony White, Box Hill (AU)
Assigned to NANOCYTOMICS, LLC, Evanston, IL (US); and NORTHWESTERN UNIVERSITY, Evanston, IL (US)
Appl. No. 17/258,873
Filed by NanoCytomics, LLC; and Northwestern University, Evanston, IL (US)
PCT Filed Jul. 10, 2019, PCT No. PCT/US2019/041150
§ 371(c)(1), (2) Date Jan. 8, 2021,
PCT Pub. No. WO2020/014323, PCT Pub. Date Jan. 16, 2020.
Claims priority of provisional application 62/696,119, filed on Jul. 10, 2018.
Prior Publication US 2021/0325412 A1, Oct. 21, 2021
Int. Cl. G01N 1/00 (2006.01); G01N 35/00 (2006.01); G01N 1/28 (2006.01); G01N 1/31 (2006.01); G01N 35/10 (2006.01)
CPC G01N 35/00029 (2013.01) [G01N 1/2813 (2013.01); G01N 1/312 (2013.01); G01N 35/1002 (2013.01); G01N 2001/317 (2013.01); G01N 2035/00039 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A substrate cartridge comprising:
(a) a spray retainer;
(b) a sample dispense port having (i) a first end of the sample dispense port for receiving a sample, (ii) a second end of the sample dispense port for releasing the sample into the spray retainer, and (iii) a sample retention zone located between the first end and the second end of the sample dispense port, wherein the second end of the sample dispense port is located at a side of the spray retainer;
(c) a reagent port for dispensing a fixative reagent into the spray retainer; and
(d) an upper opening for receiving a spray nozzle, wherein the upper opening is positioned at an angle to an opening at the second end of the sample dispense port.