US 11,719,703 B2
Mass spectrometry technique for single cell proteomics
Bogdan Budnik, Brookline, MA (US); Nikolai Slavov, Cambridge, MA (US); Harrison Specht, Arlington, MA (US); and Ezra Levy, Boston, MA (US)
Assigned to NORTHEASTERN UNIVERSITY, Boston, MA (US); and PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Filed by Northeastern University, Boston, MA (US); and President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Jan. 17, 2019, as Appl. No. 16/251,039.
Claims priority of provisional application 62/725,864, filed on Aug. 31, 2018.
Claims priority of provisional application 62/678,261, filed on May 30, 2018.
Claims priority of provisional application 62/618,301, filed on Jan. 17, 2018.
Prior Publication US 2019/0219592 A1, Jul. 18, 2019
Int. Cl. G01N 33/68 (2006.01); G01N 1/40 (2006.01)
CPC G01N 33/6848 (2013.01) [G01N 1/4022 (2013.01); G01N 2440/00 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01); G01N 2570/00 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of identifying and quantifying labeled test peptides in a test sample comprising a single cell, the method comprising:
mechanically lysing the test sample to obtain a cell lysate;
digesting proteins released from the cell lysate to obtain test peptides;
labeling the test peptides to produce labeled test peptides;
mixing the labeled test peptides from the test sample with labeled carrier peptides from a carrier sample to form a mixture, wherein the carrier sample is obtained from at least about 10-fold more cells than the test sample, and wherein the labeled test peptides and the labeled carrier peptides have different labels; and
performing liquid chromatography and tandem mass spectroscopy (LC-MS/MS) on the mixture to obtain an analysis of the labeled test peptides,
thereby identifying and quantifying the labeled test peptides.