CPC G01N 33/6848 (2013.01) [G01N 1/4022 (2013.01); G01N 2440/00 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01); G01N 2570/00 (2013.01)] | 17 Claims |
1. A method of identifying and quantifying labeled test peptides in a test sample comprising a single cell, the method comprising:
mechanically lysing the test sample to obtain a cell lysate;
digesting proteins released from the cell lysate to obtain test peptides;
labeling the test peptides to produce labeled test peptides;
mixing the labeled test peptides from the test sample with labeled carrier peptides from a carrier sample to form a mixture, wherein the carrier sample is obtained from at least about 10-fold more cells than the test sample, and wherein the labeled test peptides and the labeled carrier peptides have different labels; and
performing liquid chromatography and tandem mass spectroscopy (LC-MS/MS) on the mixture to obtain an analysis of the labeled test peptides,
thereby identifying and quantifying the labeled test peptides.
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