US 11,719,698 B2
Cancer detection method using sense of smell of nematode
Takaaki Hirotsu, Fukuoka (JP); and Hideto Sonoda, Saga (JP)
Assigned to HIROTSU BIO SCIENCE INC., Tokyo (JP)
Appl. No. 15/103,264
Filed by HIROTSU BIO SCIENCE INC., Tokyo (JP)
PCT Filed Dec. 10, 2014, PCT No. PCT/JP2014/083320
§ 371(c)(1), (2) Date Jun. 9, 2016,
PCT Pub. No. WO2015/088039, PCT Pub. Date Jun. 18, 2015.
Claims priority of provisional application 61/982,341, filed on Apr. 22, 2014.
Claims priority of application No. 2013-255145 (JP), filed on Dec. 10, 2013.
Prior Publication US 2017/0016906 A1, Jan. 19, 2017
Int. Cl. G01N 33/574 (2006.01); C12Q 1/6888 (2018.01)
CPC G01N 33/57488 (2013.01) [C12Q 1/6888 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/158 (2013.01); G01N 2333/43534 (2013.01)] 10 Claims
 
1. A method for detecting cancer in a subject, comprising the steps of:
(a) obtaining a test sample which comprises urine, a cell culture medium, or a preservative solution of the cells or tissues from the subject and wherein a concentration of the urine, the cell culture medium, or the preservative solution of the cells or tissues is diluted with a diluting liquid,
wherein the urine is diluted to 1.5 to 1000 times or the cell culture medium is diluted to 106 to 107 times, or the preservative solution of the cells or preservative solution of the tissues is diluted to 102 to 104 times;
(b) exposing Caenorhabditis elegans nematodes to the test sample and a control sample separately, and
(c) assaying a chemotaxis of the Caenorhabditis elegans nematodes to the test sample and the control sample separately,
wherein a positive chemotaxis by the Caenorhabditis elegans nematodes to the test sample indicates that the subject is determined to have cancer, or to have a risk of having cancer, and
wherein a negative chemotaxis by the Caenorhabditis elegans nematodes to the test sample indicates that the subject is not determined to have cancer, or have the risk of having cancer.