US 11,719,692 B2
Devices and methods of cell capture and analysis
Stephen D. Mikolajczyk, San Diego, CA (US); Tony Pircher, San Diego, CA (US); Pavel Tsinberg, San Diego, CA (US); and Farideh Z. Bischoff, San Diego, CA (US)
Assigned to BIOCEPT, INC., San Diego, CA (US)
Filed by BIOCEPT, INC., San Diego, CA (US)
Filed on Nov. 26, 2019, as Appl. No. 16/695,976.
Application 14/812,498 is a division of application No. 12/730,738, filed on Mar. 24, 2010, granted, now 9,128,082, issued on Sep. 8, 2015.
Application 16/695,976 is a continuation of application No. 14/812,498, filed on Jul. 29, 2015, granted, now 10,527,611.
Claims priority of provisional application 61/298,871, filed on Jan. 27, 2010.
Claims priority of provisional application 61/235,615, filed on Aug. 20, 2009.
Claims priority of provisional application 61/163,009, filed on Mar. 24, 2009.
Prior Publication US 2020/0340985 A1, Oct. 29, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/536 (2006.01); G01N 33/569 (2006.01); G01N 33/543 (2006.01); G01N 33/574 (2006.01)
CPC G01N 33/54306 (2013.01) [G01N 33/536 (2013.01); G01N 33/56966 (2013.01); G01N 33/56972 (2013.01); G01N 33/57492 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for detecting a target in a sample comprising:
(a) pre-incubating the sample with a first binding entity and a third binding entity to form a pre-loading mixture,
wherein step (a) results in the first binding entity being specifically bound to a target entity on the surface of the target and the first binding entity being specifically bound to the third binding entity, and
wherein the first binding entity is not conjugated to a detectable or capturable entity,
(b) contacting the pre-loading mixture with a surface, wherein the surface is coated with a second binding entity capable of specifically binding, directly or indirectly, to the third binding entity, wherein step (b) results in the binding of the second binding entity to the third binding entity and thereby in capture of the target on the surface,
wherein the second binding entity is not an antibody, and
(c) detecting the presence of the captured target on the surface.
 
14. A method for capturing a target cell in a biological sample comprising:
(a) pre-incubating the biological sample with a first binding entity and a third binding entity to form a pre-loading mixture, wherein step (a) results in the first binding entity being specifically bound to a target entity on the surface of the target cell and in the first binding entity being specifically bound to the third binding entity, and
wherein the first binding entity is not conjugated to a detectable or capturable entity,
(b) contacting the pre-loading mixture with a surface, wherein the surface is coated with a second binding entity capable of specifically binding, directly or indirectly, to the third binding entity wherein step (b) results in the binding of the second binding entity to the third binding entity and thereby in capture of the target cell on the surface,
wherein the second binding entity is not an antibody.