US 11,719,690 B2
Methods for extracting A1P1 and albumin from blood plasma
Eugene Zurlo, Charleston, SC (US); and Dennis Curtin, Plattsburgh, NY (US)
Assigned to Plasma Technologies, LLC, Charleston, SC (US)
Filed by Plasma Technologies, LLC, Charleston, SC (US)
Filed on Sep. 23, 2020, as Appl. No. 17/30,167.
Application 17/030,167 is a continuation of application No. 15/767,639, granted, now 10,809,247, previously published as PCT/US2016/057196, filed on Oct. 14, 2016.
Claims priority of provisional application 62/242,165, filed on Oct. 15, 2015.
Prior Publication US 2021/0003552 A1, Jan. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01D 15/12 (2006.01); B01D 15/38 (2006.01); C07K 1/18 (2006.01); C07K 1/22 (2006.01); C07K 1/32 (2006.01); C07K 14/81 (2006.01); G01N 30/02 (2006.01); G01N 33/49 (2006.01); G01N 30/88 (2006.01)
CPC G01N 33/491 (2013.01) [B01D 15/12 (2013.01); B01D 15/3804 (2013.01); C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 1/32 (2013.01); C07K 14/81 (2013.01); C07K 14/8125 (2013.01); G01N 30/02 (2013.01); G01N 2030/8822 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of separating A1P1 from a blood plasma containing product, the method comprising:
thawing frozen blood plasma from a plasma source followed by mixing the frozen blood plasma with cryoprecipitate resulting from the thawing to generate a blood plasma containing product;
adding a salt to the blood plasma containing product to produce a first intermediate, wherein the salt comprises between 11-20 wt % of the first intermediate;
separating the first intermediate to produce a first supernatant and a first paste;
adding a salt to the first supernatant to produce a second intermediate, wherein the salt comprises between 15-30 wt % of the second intermediate;
separating the second intermediate to produce a second supernatant and a second paste; and
separating the second supernatant by affinity chromatography using an A1P1-specific affinity media to generate a flow-through fraction and a first eluate, wherein the first eluate comprises A1P1.