CPC G01N 33/491 (2013.01) [B01D 15/12 (2013.01); B01D 15/3804 (2013.01); C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 1/32 (2013.01); C07K 14/81 (2013.01); C07K 14/8125 (2013.01); G01N 30/02 (2013.01); G01N 2030/8822 (2013.01)] | 20 Claims |
1. A method of separating A1P1 from a blood plasma containing product, the method comprising:
thawing frozen blood plasma from a plasma source followed by mixing the frozen blood plasma with cryoprecipitate resulting from the thawing to generate a blood plasma containing product;
adding a salt to the blood plasma containing product to produce a first intermediate, wherein the salt comprises between 11-20 wt % of the first intermediate;
separating the first intermediate to produce a first supernatant and a first paste;
adding a salt to the first supernatant to produce a second intermediate, wherein the salt comprises between 15-30 wt % of the second intermediate;
separating the second intermediate to produce a second supernatant and a second paste; and
separating the second supernatant by affinity chromatography using an A1P1-specific affinity media to generate a flow-through fraction and a first eluate, wherein the first eluate comprises A1P1.
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