US 11,719,608 B2
Method for processing tissue samples
Stevan Bogdan Jovanovich, Livermore, CA (US); Frank Zaugg, Redwood City, CA (US); Kaiwan Chear, Livermore, CA (US); Roger McIntosh, San Ramon, CA (US); and Nathan Pereira, Pleasanton, CA (US)
Assigned to S2 GENOMICS, INC., Livermore, CA (US)
Filed by S2 Genomics, Inc., Livermore, CA (US)
Filed on Jan. 23, 2022, as Appl. No. 17/581,940.
Application 17/581,940 is a continuation of application No. 17/513,204, filed on Oct. 28, 2021, granted, now 11,441,976.
Application 17/513,204 is a continuation of application No. 16/301,249, granted, now 11,231,347, previously published as PCT/US2017/063811, filed on Nov. 29, 2017.
Claims priority of provisional application 62/526,267, filed on Jun. 28, 2017.
Claims priority of provisional application 62/427,150, filed on Nov. 29, 2016.
Prior Publication US 2022/0146382 A1, May 12, 2022
Int. Cl. G01N 1/31 (2006.01); C12M 1/00 (2006.01); C12Q 1/6851 (2018.01); C12N 1/06 (2006.01); C12N 15/10 (2006.01); G01N 1/28 (2006.01)
CPC G01N 1/31 (2013.01) [C12M 47/04 (2013.01); C12M 47/06 (2013.01); C12N 1/06 (2013.01); C12N 1/066 (2013.01); C12N 15/1003 (2013.01); C12Q 1/6851 (2013.01); G01N 1/286 (2013.01); G01N 2001/2866 (2013.01)] 39 Claims
OG exemplary drawing
 
1. A method of preparing a sample, comprising:
(a) introducing a tissue or cell sample into a chamber such that the tissue or cell sample is in a liquid medium that is in contact with a non-porous grinding stator within the chamber;
(b) introducing a tissue disruptor comprising disruptor grinding features that protrude from a surface of the tissue disruptor into the chamber;
(c) performing mechanical disruption of the tissue or cell sample in the chamber, wherein the mechanical disruption is conducted by grinding the tissue or cell sample between the disruptor grinding features and the grinding stator to produce (i) released cells or released subcellular organelles and (ii) debris;
(d) separating the released cells or the released subcellular organelles from a portion of the debris; and
(e) collecting the released cells or released subcellular organelles in a liquid suspension.