CPC G01N 1/31 (2013.01) [C12M 47/04 (2013.01); C12M 47/06 (2013.01); C12N 1/06 (2013.01); C12N 1/066 (2013.01); C12N 15/1003 (2013.01); C12Q 1/6851 (2013.01); G01N 1/286 (2013.01); G01N 2001/2866 (2013.01)] | 39 Claims |
1. A method of preparing a sample, comprising:
(a) introducing a tissue or cell sample into a chamber such that the tissue or cell sample is in a liquid medium that is in contact with a non-porous grinding stator within the chamber;
(b) introducing a tissue disruptor comprising disruptor grinding features that protrude from a surface of the tissue disruptor into the chamber;
(c) performing mechanical disruption of the tissue or cell sample in the chamber, wherein the mechanical disruption is conducted by grinding the tissue or cell sample between the disruptor grinding features and the grinding stator to produce (i) released cells or released subcellular organelles and (ii) debris;
(d) separating the released cells or the released subcellular organelles from a portion of the debris; and
(e) collecting the released cells or released subcellular organelles in a liquid suspension.
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