CPC C40B 40/08 (2013.01) [C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C07K 16/005 (2013.01); C07K 16/2887 (2013.01); C07K 16/2896 (2013.01); C12N 15/85 (2013.01); G01N 33/502 (2013.01); G01N 33/505 (2013.01); G01N 33/5011 (2013.01); G01N 33/5052 (2013.01); C07K 2317/622 (2013.01); C07K 2319/03 (2013.01); C07K 2319/33 (2013.01); C07K 2319/70 (2013.01); C40B 30/04 (2013.01); G01N 2500/10 (2013.01)] | 10 Claims |
1. A plurality of nucleic acids comprising: a plurality of polynucleotides encoding a plurality of different antigen binding domains that are derived from a subject, wherein the subject has an immune response to a cancer; wherein the each individual member of the plurality of polynucleotides encodes a fusion polypeptide comprising the antigen binding domain fused to a CAR chassis, wherein the CAR chassis is comprised of a transmembrane element and an intracellular element comprising a T cell receptor zeta chain and a costimulatory molecule, wherein the transmembrane element is fused to the intracellular element and the costimulatory molecule is fused to the intracellular element, wherein the costimulatory molecule is CD137, CD27 or CD28.
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