US 11,718,929 B2
Methods for making novel antigen binding domains
Benjamin Wang, Menlo Park, CA (US); and Gusti Zeiner, Pacifica, CA (US)
Assigned to Chimera Bioengineering, Inc.
Filed by Chimera Bioengineering, Inc., Menlo Park, CA (US)
Filed on Jul. 19, 2021, as Appl. No. 17/379,514.
Application 17/379,514 is a division of application No. 16/275,746, filed on Feb. 14, 2019, granted, now 11,085,917.
Application 16/275,746 is a continuation of application No. 15/596,493, filed on May 16, 2017, granted, now 10,222,369, issued on Mar. 5, 2019.
Claims priority of provisional application 62/337,769, filed on May 17, 2016.
Prior Publication US 2021/0341463 A1, Nov. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C40B 40/08 (2006.01); C07K 16/00 (2006.01); C12N 15/85 (2006.01); C07K 16/28 (2006.01); C07K 14/725 (2006.01); C07K 14/705 (2006.01); G01N 33/50 (2006.01); C40B 30/04 (2006.01)
CPC C40B 40/08 (2013.01) [C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C07K 16/005 (2013.01); C07K 16/2887 (2013.01); C07K 16/2896 (2013.01); C12N 15/85 (2013.01); G01N 33/502 (2013.01); G01N 33/505 (2013.01); G01N 33/5011 (2013.01); G01N 33/5052 (2013.01); C07K 2317/622 (2013.01); C07K 2319/03 (2013.01); C07K 2319/33 (2013.01); C07K 2319/70 (2013.01); C40B 30/04 (2013.01); G01N 2500/10 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A plurality of nucleic acids comprising: a plurality of polynucleotides encoding a plurality of different antigen binding domains that are derived from a subject, wherein the subject has an immune response to a cancer; wherein the each individual member of the plurality of polynucleotides encodes a fusion polypeptide comprising the antigen binding domain fused to a CAR chassis, wherein the CAR chassis is comprised of a transmembrane element and an intracellular element comprising a T cell receptor zeta chain and a costimulatory molecule, wherein the transmembrane element is fused to the intracellular element and the costimulatory molecule is fused to the intracellular element, wherein the costimulatory molecule is CD137, CD27 or CD28.