CPC C12Q 1/6893 (2013.01) [C12Q 2600/16 (2013.01)] | 2 Claims |
1. A kit for detecting a nucleic acid of the Babesia species of B. microti, B. divergens, B. duncani, and/or B. venatorum that may be present in a sample, wherein the kit comprises amplification reagents comprising a DNA polymerase, nucleotide monomers, and one or more set of primers and one or more probes, wherein the one or more set of primers and the one or more probes comprise:
(1) a set of primers for amplification of B. microti comprising: (i) a primer comprising the nucleic acid sequence of SEQ ID NO:1, or a complement thereof, and (ii) a primer comprising the nucleic acid sequence of SEQ ID NO:3, or a complement thereof, and a probe for hybridizing to the amplification product of B. microti comprising the nucleic acid sequence of SEQ ID NO:2, or a complement thereof, and
(2) a set of primers for amplification of B. divergens, B. duncani, and/or B. venatorum comprising: (i) a primer comprising the nucleic acid sequence of SEQ ID NO:4, or a complement thereof, and (ii) one or more primers comprising the nucleic acid sequence(s) of SEQ ID NO:6 or SEQ ID NO:7 or a combination of SEQ ID NOs:6 and 7, or a complement thereof; and a probe for hybridizing to the amplification product of B. divergens, B. duncani, and/or B. venatorum comprising the nucleic acid sequence of SEQ ID NO:5, or a complement thereof.
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