US 11,718,881 B2
Detection reagent, detection kit and detection method for ITGA4 gene methylation
Xianglin Liu, Guangdong (CN); and Hongzhi Zou, Guangdong (CN)
Assigned to CREATIVE BIOSCIENCES (GUANGZHOU) CO., LTD., Guangdong (CN)
Appl. No. 16/603,451
Filed by CREATIVE BIOSCIENCES (GUANGZHOU) CO., LTD., Guangdong (CN)
PCT Filed Dec. 26, 2017, PCT No. PCT/CN2017/118563
§ 371(c)(1), (2) Date Oct. 7, 2019,
PCT Pub. No. WO2018/188377, PCT Pub. Date Oct. 18, 2018.
Claims priority of application No. 201710236243.9 (CN), filed on Apr. 12, 2017.
Prior Publication US 2020/0149116 A1, May 14, 2020
Int. Cl. C12Q 1/6886 (2018.01); G01N 33/543 (2006.01)
CPC C12Q 1/6886 (2013.01) [G01N 33/54326 (2013.01)] 4 Claims
 
1. A colorectal cancer detection kit for use on excrement sample, comprising:
an ITGA4 gene capturing reagent, comprising a magnetic bead probe complex; and
an ITGA4 gene methylation detection reagent, comprising:
a primer pair of nucleotide sequences consisting of SEQ ID NO: 4 and SEQ ID NO:5 respectively; and
a probe consisting of SEQ ID NO: 6.