US 11,718,868 B2
Nanoparticle-based lipase biosensor utilizing a custom-synthesized peptidyl-ester substrate
Igor Medintz, Washington, DC (US); Joyce A. Breger, Washington, DC (US); Kimihiro Susumu, Washington, DC (US); Sebastian Diaz, Washington, DC (US); and Jesper Brask, Lyngby (DK)
Assigned to The Government of the United States of America, as represented by the Secretary of the Navy, Arlington, VA (US)
Filed by The Government of the United States of America, as represented by the Secretary of the Navy, Arlington, VA (US)
Filed on Jan. 29, 2021, as Appl. No. 17/161,889.
Claims priority of provisional application 62/968,746, filed on Jan. 31, 2020.
Prior Publication US 2021/0238650 A1, Aug. 5, 2021
Int. Cl. C12Q 1/44 (2006.01); C12Q 1/34 (2006.01); C09K 11/56 (2006.01); G01N 21/64 (2006.01); C09K 11/88 (2006.01)
CPC C12Q 1/44 (2013.01) [C09K 11/565 (2013.01); C09K 11/883 (2013.01); C12Q 1/34 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01)] 7 Claims
 
1. A biosensor comprising a quantum dot (QD) and at least one construct adhered to the QD, wherein the construct comprises a peptide, a fluorophore acceptor configured as a Förster resonance energy transfer (FRET) acceptor to the QD and a cleavable ester positioned between the peptide and the fluorophore acceptor, wherein the peptide comprises an amino acid sequence of SEQ ID NO:1, including a nanoparticle association domain effective to bind the construct to the QD; the ester comprises

OG Complex Work Unit Chemistry
and wherein cleavage of the ester results in dissociation of the QD and the fluorophore acceptor and thus a change in FRET activity.