CPC C12Q 1/44 (2013.01) [C09K 11/565 (2013.01); C09K 11/883 (2013.01); C12Q 1/34 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01)] | 7 Claims |
1. A biosensor comprising a quantum dot (QD) and at least one construct adhered to the QD, wherein the construct comprises a peptide, a fluorophore acceptor configured as a Förster resonance energy transfer (FRET) acceptor to the QD and a cleavable ester positioned between the peptide and the fluorophore acceptor, wherein the peptide comprises an amino acid sequence of SEQ ID NO:1, including a nanoparticle association domain effective to bind the construct to the QD; the ester comprises
and wherein cleavage of the ester results in dissociation of the QD and the fluorophore acceptor and thus a change in FRET activity.
|