US 11,718,866 B2
Engineered water nanostructures (EWNS) and uses thereof
Philip Demokritou, Brookline, MA (US); Georgios Pyrgiotakis, Cranford, NJ (US); and Joseph D. Brain, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Aug. 2, 2022, as Appl. No. 17/879,029.
Application 17/879,029 is a division of application No. 15/511,532, granted, now 11,479,799, previously published as PCT/US2015/050460, filed on Sep. 16, 2015.
Claims priority of provisional application 62/050,996, filed on Sep. 16, 2014.
Prior Publication US 2023/0036792 A1, Feb. 2, 2023
Int. Cl. C12Q 1/02 (2006.01); A61L 2/00 (2006.01); A61L 2/03 (2006.01); A61L 2/22 (2006.01); A61L 9/14 (2006.01); A61L 9/22 (2006.01); C25B 9/00 (2021.01); A23L 3/3481 (2006.01); A23L 3/3409 (2006.01); A23L 3/32 (2006.01); A23L 3/3463 (2006.01); C12N 1/20 (2006.01); C02F 1/46 (2023.01)
CPC C12Q 1/02 (2013.01) [A23L 3/32 (2013.01); A23L 3/3409 (2013.01); A23L 3/3463 (2013.01); A23L 3/3481 (2013.01); A61L 2/00 (2013.01); A61L 2/03 (2013.01); A61L 2/22 (2013.01); A61L 9/14 (2013.01); A61L 9/22 (2013.01); C12N 1/20 (2013.01); C25B 9/00 (2013.01); A61L 2/0088 (2013.01); A61L 2202/11 (2013.01); A61L 2209/213 (2013.01); C02F 1/4608 (2013.01); C02F 2303/04 (2013.01); C02F 2305/023 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for inactivating at least one of bacteria, mycobacteria, bacterial spores, and fungi on foodstuffs comprising:
contacting the foodstuffs with engineered water nanostructures (EWNS) comprising reactive oxygen species (ROS) and an electric charge from about 20 to about 100 e−; wherein the electric charge of the EWNS is an average EWNS electric charge of a sample of EWNS, the average EWNS electric charge determinable by parallel measurement of the aerosol current and the particle number concentration of the sample of EWNS and the contacting inactivates the at least one of bacteria, mycobacteria, bacterial spores, and fungi located on the foodstuffs.