CPC C12N 15/86 (2013.01) [C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2840/203 (2013.01)] | 28 Claims |
1. An adeno-associated virus (AAV) genome encoding a circular RNA, wherein the AAV genome comprises, from 5′ to 3′:
(a) a first inverted terminal repeat;
(b) a first intronic element;
(c) a nucleotide sequence of interest;
(d) a second intronic element;
(e) a second inverted terminal repeat;
wherein (c) is directly coupled to (b) and (d), respectively; and
wherein the first intronic element and the second intronic element are at least one pair selected from the group consisting of:
the first intronic element consists of SEQ ID NO:13 and the second intronic element comprises SEQ ID NO:14;
the first intronic element consists of SEQ ID NO:15 and the second intronic element comprises SEQ ID NO:16;
the first intronic element consists of SEQ ID NO:15 and the second intronic the first intronic element consists of SEQ ID NO:15 and the second intronic element comprises SEQ ID NO:18;
the first intronic element consists of SEQ ID NO:17 and the second intronic element comprises SEQ ID NO:18;
the first intronic element consists of SEQ ID NO:17 and the second intronic the first intronic element consists of SEQ ID NO:17 and the second intronic element comprises SEQ ID NO:16;
the first intronic element consists of SEQ ID NO:19 and the second intronic element comprises SEQ ID NO:20;
the first intronic element consists of SEQ ID NO:21 and the second intronic element comprises SEQ ID NO:22;
the first intronic element consists of SEQ ID NO:23 and the second intronic element comprises SEQ ID NO:24;
the first intronic element consists of SEQ ID NO:29 and the second intronic element comprises SEQ ID NO:30; and
the first intronic element consists of SEQ ID NO:31 and the second intronic element comprises SEQ ID NO:32.
|