US 11,718,862 B2
Methods and compositions for circular RNA molecules
Aravind Asokan, Chapel Hill, NC (US); Erin Borchardt, Naperville, IL (US); Rita Meganck, Raleigh, NC (US); and William F. Marzluff, Hillsborough, NC (US)
Assigned to The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed by The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed on Oct. 20, 2021, as Appl. No. 17/506,089.
Application 17/506,089 is a continuation of application No. 16/761,876, previously published as PCT/US2018/059652, filed on Nov. 7, 2018.
Claims priority of provisional application 62/582,796, filed on Nov. 7, 2017.
Prior Publication US 2022/0090137 A1, Mar. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01); C12N 15/00 (2006.01); C12N 15/63 (2006.01); C12N 15/86 (2006.01); A01K 67/00 (2006.01)
CPC C12N 15/86 (2013.01) [C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2840/203 (2013.01)] 28 Claims
 
1. An adeno-associated virus (AAV) genome encoding a circular RNA, wherein the AAV genome comprises, from 5′ to 3′:
(a) a first inverted terminal repeat;
(b) a first intronic element;
(c) a nucleotide sequence of interest;
(d) a second intronic element;
(e) a second inverted terminal repeat;
wherein (c) is directly coupled to (b) and (d), respectively; and
wherein the first intronic element and the second intronic element are at least one pair selected from the group consisting of:
the first intronic element consists of SEQ ID NO:13 and the second intronic element comprises SEQ ID NO:14;
the first intronic element consists of SEQ ID NO:15 and the second intronic element comprises SEQ ID NO:16;
the first intronic element consists of SEQ ID NO:15 and the second intronic the first intronic element consists of SEQ ID NO:15 and the second intronic element comprises SEQ ID NO:18;
the first intronic element consists of SEQ ID NO:17 and the second intronic element comprises SEQ ID NO:18;
the first intronic element consists of SEQ ID NO:17 and the second intronic the first intronic element consists of SEQ ID NO:17 and the second intronic element comprises SEQ ID NO:16;
the first intronic element consists of SEQ ID NO:19 and the second intronic element comprises SEQ ID NO:20;
the first intronic element consists of SEQ ID NO:21 and the second intronic element comprises SEQ ID NO:22;
the first intronic element consists of SEQ ID NO:23 and the second intronic element comprises SEQ ID NO:24;
the first intronic element consists of SEQ ID NO:29 and the second intronic element comprises SEQ ID NO:30; and
the first intronic element consists of SEQ ID NO:31 and the second intronic element comprises SEQ ID NO:32.