	US 11,718,858 B2
	Biocontainment/biocontrol system and methods
Maciej Maselko, Falcon Heights, MN (US); and Mike Smanski, Falcon Heights, MN (US)
Assigned to REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
Appl. No. 15/775,164
Filed by REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
PCT Filed Nov. 10, 2016, PCT No. PCT/US2016/061297 § 371(c)(1), (2) Date May 10, 2018, PCT Pub. No. WO2017/083501, PCT Pub. Date May 18, 2017.
Claims priority of provisional application 62/253,954, filed on Nov. 11, 2015.
Prior Publication US 2018/0327762 A1, Nov. 15, 2018
Int. Cl. C12N 15/63 (2006.01); C12N 9/22 (2006.01); C12N 15/82 (2006.01); C12N 15/81 (2006.01); C12N 1/14 (2006.01); C12N 1/18 (2006.01); C12N 15/80 (2006.01); C07K 14/005 (2006.01)

CPC C12N 15/8218 (2013.01) [C12N 1/14 (2013.01); C12N 1/18 (2013.01); C12N 9/22 (2013.01); C12N 15/63 (2013.01); C12N 15/80 (2013.01); C12N 15/81 (2013.01); C12N 15/815 (2013.01); C12N 15/8216 (2013.01); C12N 15/8217 (2013.01); C12N 15/8263 (2013.01); C12N 15/8265 (2013.01); C07K 14/005 (2013.01); C07K 2319/00 (2013.01); C12N 2510/00 (2013.01)]

18 Claims

1. A cell of a sexually reproducing organism, the cell comprising a biocontainment system that comprises:

a coding region whose overexpression alters growth of the cell or impairs cell division;

a transcription regulatory region operably linked upstream of the coding region, and comprising a silent mutation; and

a polynucleotide that encodes a programmable transcription activator engineered to bind to the transcription regulatory region in the absence of the silent mutation after sexual reproduction, thereby overexpressing the coding region in the absence of the silent mutation, but does not initiate overexpression of the coding region when the transcription regulatory region comprises the silent mutation.