US 11,718,855 B2
Methods for producing transgenic plants
Anisha Akula, Madison, WI (US); David R. Duncan, Saint Charles, MO (US); Brenda A. Lowe, Mystic, CT (US); Michael T. Mann, Sacramento, CA (US); William L. Petersen, Roxbury, WI (US); Jyoti R. Rout, Portland, OR (US); David D. Songstad, Ballwin, MO (US); Joel B. Wilks, East Hampton, CT (US); and Wanggen Zhang, Wildwood, MO (US)
Assigned to MONSANTO TECHNOLOGY, LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Jul. 8, 2021, as Appl. No. 17/370,598.
Application 17/370,598 is a division of application No. 15/977,265, filed on May 11, 2018, granted, now 11,091,766.
Application 15/977,265 is a division of application No. 14/598,744, filed on Jan. 16, 2015, granted, now 10,006,036, issued on Jun. 26, 2018.
Application 14/598,744 is a division of application No. 13/945,727, filed on Jul. 18, 2013, granted, now 8,962,326, issued on Feb. 24, 2015.
Application 13/945,727 is a division of application No. 13/355,312, filed on Jan. 20, 2012, granted, now 8,513,016, issued on Aug. 20, 2013.
Application 13/355,312 is a division of application No. 11/848,554, filed on Aug. 31, 2007, granted, now 8,124,411, issued on Feb. 28, 2012.
Claims priority of provisional application 60/841,519, filed on Aug. 31, 2006.
Prior Publication US 2022/0010320 A1, Jan. 13, 2022
Int. Cl. C12N 15/82 (2006.01); A01H 4/00 (2006.01); A01H 1/04 (2006.01); A01H 6/46 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6813 (2018.01); C12Q 1/25 (2006.01); G01N 33/53 (2006.01)
CPC C12N 15/8201 (2013.01) [A01H 1/04 (2013.01); A01H 4/001 (2013.01); A01H 6/4684 (2018.05); C12N 15/821 (2013.01); C12N 15/8209 (2013.01); C12N 15/8212 (2013.01); C12N 15/8265 (2013.01); C12N 15/8205 (2013.01); C12N 15/8261 (2013.01); C12Q 1/25 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6813 (2013.01); G01N 33/53 (2013.01)] 9 Claims
 
1. A method of obtaining an explant for producing a transgenic corn plant comprising dividing a callus into smaller callus pieces, wherein the smaller callus pieces are fitted into a multi-well plate, wherein the callus is a type I callus.