US 11,718,854 B2
Gene targets for improved enzyme production in fungi
Scott E. Baker, Richland, WA (US); Jon K. Magnuson, Richland, WA (US); Morgann C. Reilly, Cambridge, MA (US); Joonhoon Kim, Berkeley, CA (US); John Gladden, Alameda, CA (US); and Jed J. Lynn, Fleming Island, FL (US)
Assigned to Battelle Memorial Institute, Richland, WA (US); and National Technology & Engineering Solutions of Sandia, LLC, Albuquerque, NM (US)
Filed by Battelle Memorial Institute, Richland, WA (US); and National Technology & Engineering Solutions of Sandia, LLC, Albuquerque, NM (US)
Filed on Jan. 28, 2021, as Appl. No. 17/161,502.
Application 17/161,502 is a division of application No. 16/163,509, filed on Oct. 17, 2018, granted, now 10,934,551.
Claims priority of provisional application 62/573,354, filed on Oct. 17, 2017.
Prior Publication US 2021/0254077 A1, Aug. 19, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/15 (2006.01); C12N 9/34 (2006.01); C12N 15/80 (2006.01); C12N 15/52 (2006.01); C12P 21/00 (2006.01); C12P 19/14 (2006.01); C12N 9/42 (2006.01); C12N 9/20 (2006.01); C12N 9/50 (2006.01); C07K 14/38 (2006.01); C12N 9/24 (2006.01); C12P 21/02 (2006.01); C07K 14/37 (2006.01); C12N 1/14 (2006.01); C12R 1/685 (2006.01)
CPC C12N 15/52 (2013.01) [C07K 14/37 (2013.01); C07K 14/38 (2013.01); C12N 1/145 (2021.05); C12N 9/20 (2013.01); C12N 9/2402 (2013.01); C12N 9/2428 (2013.01); C12N 9/2437 (2013.01); C12N 9/2445 (2013.01); C12N 9/50 (2013.01); C12N 15/80 (2013.01); C12P 21/02 (2013.01); C12Y 302/0102 (2013.01); C12R 2001/685 (2021.05); C12Y 301/01001 (2013.01); C12Y 301/01003 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01021 (2013.01); C12Y 302/01176 (2013.01); C12Y 304/21 (2013.01)] 32 Claims
 
1. An isolated Aspergillus fungus, comprising:
a genetic inactivation of a mstC gene encoding a polypeptide comprising monosaccharide transporter activity and an amino acid sequence with at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2, and
a polynucleotide comprising a glucoamylase promoter operably linked to a heterologous protein coding sequence encoding a heterologous protein,
wherein said heterologous protein coding sequence is heterologous to the isolated Aspergillus fungus, and wherein said heterologous protein coding sequence is heterologous or homologous to the glucoamylase promoter.