CPC C12N 15/52 (2013.01) [C07K 14/37 (2013.01); C07K 14/38 (2013.01); C12N 1/145 (2021.05); C12N 9/20 (2013.01); C12N 9/2402 (2013.01); C12N 9/2428 (2013.01); C12N 9/2437 (2013.01); C12N 9/2445 (2013.01); C12N 9/50 (2013.01); C12N 15/80 (2013.01); C12P 21/02 (2013.01); C12Y 302/0102 (2013.01); C12R 2001/685 (2021.05); C12Y 301/01001 (2013.01); C12Y 301/01003 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01021 (2013.01); C12Y 302/01176 (2013.01); C12Y 304/21 (2013.01)] | 32 Claims |
1. An isolated Aspergillus fungus, comprising:
a genetic inactivation of a mstC gene encoding a polypeptide comprising monosaccharide transporter activity and an amino acid sequence with at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2, and
a polynucleotide comprising a glucoamylase promoter operably linked to a heterologous protein coding sequence encoding a heterologous protein,
wherein said heterologous protein coding sequence is heterologous to the isolated Aspergillus fungus, and wherein said heterologous protein coding sequence is heterologous or homologous to the glucoamylase promoter.
|