CPC C12N 15/1082 (2013.01) [C12N 15/1093 (2013.01)] | 7 Claims |
1. A method of preparing a library of phosphopeptide-encoding oligonucleotides, the method comprising:
selecting a plurality of phosphopeptide sequences from full-length native protein sequences, comprising:
identifying, in a full-length native protein sequence, a plurality of phosphorylation-susceptible amino acid sequences, wherein each phosphorylation-susceptible amino acid sequence comprises a single phosphorylation-susceptible residue;
elongating each phosphorylation-susceptible amino acid sequence to include up to 15 residues from the full-length native protein sequence on each side of the single phosphorylation-susceptible residue, wherein each phosphopeptide sequence, of the plurality of phosphopeptide sequences, comprises 16 to 31 residues;
in silico converting each phosphopeptide sequence, of the selected plurality of phosphopeptide sequences, into a phosphopeptide-encoding nucleic acid sequence, wherein the single phosphorylation-susceptible residue is encoded as a codon that does not encode a phosphorylation-residue in nature, and wherein the single phosphorylation-susceptible residue is encoded by a codon of thymine, adenine, and/or guanine; and
synthesizing a library of phosphopeptide-encoding oligonucleotides having the phosphopeptide-encoding nucleic acid sequences, wherein each phosphopeptide-encoding oligonucleotide, of the library of phosphopeptide-encoding oligonucleotides has one of the phosphopeptide-encoding nucleic acid sequences.
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