US 11,718,840 B2
Method for screening alpha-amylases
Svend Kaasgaard, Skovlunde (DK); Signe Eskildsen Larsen, Lyngby (DK); Jens Oebro, Humlebaek (DK); Lars Beier, Soeborg (DK); Connie Pontoppidan, Lyngby (DK); Iben Damager, Valby (DK); Carsten Andersen, Vaerloese (DK); and Allan Svendsen, Hoersholm (DK)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
Filed on Aug. 5, 2019, as Appl. No. 16/531,247.
Application 16/531,247 is a continuation of application No. 14/127,781, abandoned, previously published as PCT/EP2012/062785, filed on Jun. 29, 2012.
Claims priority of provisional application 61/503,772, filed on Jul. 1, 2011.
Claims priority of application No. 11172286 (EP), filed on Jun. 30, 2011.
Prior Publication US 2019/0352628 A1, Nov. 21, 2019
Int. Cl. C12N 9/28 (2006.01); C12N 9/26 (2006.01); C12Q 1/40 (2006.01)
CPC C12N 9/2417 (2013.01) [C12N 9/2414 (2013.01); C12Q 1/40 (2013.01)] 14 Claims
 
1. A method for screening variant alpha-amylases of a parent alpha-amylase of SEQ ID NO: 2, comprising the steps of
a) determining the binding of the alpha-amylases to a solid or an immobilized substrate for the alpha amylase; and
b) selecting variant alpha-amylases having a lower binding to solid starch and higher wash performance at low temperature compared with the parent alpha-amylase, wherein the variant alpha-amylases have at least 80% sequence identity but less than 100% sequence identity to SEQ ID NO: 2 and comprise a deletion of positions 181+182, 182+183, or 183+184, and further comprise one or two or more substitutions in any of positions corresponding to W159, W167, Q169, W189, E194, N260, F262, W284, F289, G304, G305, W347, W469, G476 and G477 in SEQ ID NO: 2.