	US 11,718,837 B2
	Lipase and uses of the same
Tomohiro Kimura, Kakamigahara (JP); Tomoya Ryoki, Kakamigahara (JP); Yusuke Tanaka, Kakamigahara (JP); Naoto Katsumata, Kakamigahara (JP); and Yukihide Sato, Kakamigahara (JP)
Assigned to AMANO ENZYME INC., Nagoya (JP)
Appl. No. 17/255,831
Filed by AMANO ENZYME INC., Nagoya (JP)
PCT Filed Jul. 29, 2020, PCT No. PCT/JP2020/029104 § 371(c)(1), (2) Date Dec. 23, 2020, PCT Pub. No. WO2021/020458, PCT Pub. Date Feb. 4, 2021.
Claims priority of application No. 2019-142145 (JP), filed on Aug. 1, 2019.
Prior Publication US 2021/0214696 A1, Jul. 15, 2021
Int. Cl. C12N 9/20 (2006.01); C12P 7/64 (2022.01); C12P 7/6454 (2022.01)

CPC C12N 9/20 (2013.01) [C12P 7/64 (2013.01); C12P 7/6454 (2013.01); C12Y 301/01003 (2013.01)]

8 Claims

1. A method for manufacturing a non-site-specific transesterified oil or fat, comprising:

an enzymatic reaction with a lipase comprising an amino acid sequence having 90% or more identity with the amino acid sequence of SEQ ID NO: 1 or 2 in the presence of an acceptor substrate and a donor substrate,

wherein the acceptor substrate is a first oil or fat, glycerin fatty acid ester, or glycerin, and the donor substrate is a fatty acid, an ester compound, or a second oil or fat.