	US 11,718,832 B2
	Method to reduce oncogenic potential of induced pluripotent stem cells from aged donors
Kitai Kim, New York, NY (US); Maria Skamagki, New York, NY (US); and Yildirim Dogan, New York, NY (US)
Assigned to Memorial Sloan-Kettering Cancer Center, New York, NY (US)
Appl. No. 15/517,014
Filed by MEMORIAL SLOAN-KETTERING CANCER CENTER, New York, NY (US)
PCT Filed Oct. 6, 2015, PCT No. PCT/US2015/054319 § 371(c)(1), (2) Date Apr. 5, 2017, PCT Pub. No. WO2016/057574, PCT Pub. Date Apr. 14, 2016.
Claims priority of provisional application 62/060,532, filed on Oct. 6, 2014.
Claims priority of provisional application 62/121,463, filed on Feb. 26, 2015.
Claims priority of provisional application 62/121,460, filed on Feb. 26, 2015.
Prior Publication US 2018/0282701 A1, Oct. 4, 2018
Int. Cl. C12N 5/074 (2010.01); C07K 14/47 (2006.01); C12N 5/10 (2006.01); C12N 15/85 (2006.01)

CPC C12N 5/0696 (2013.01) [C07K 14/4702 (2013.01); C12N 5/10 (2013.01); C12N 15/85 (2013.01); C12N 2501/50 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/605 (2013.01); C12N 2501/606 (2013.01); C12N 2501/608 (2013.01); C12N 2501/71 (2013.01); C12N 2501/998 (2013.01); C12N 2510/00 (2013.01)]

15 Claims

1. A method for producing induced pluripotent stem cells generated from somatic cells of aged donors (A-iPSCs), the method comprising: supplementing somatic cells of aged donors prior to the initiation of reprogramming, during reprogramming, and/or after reprogramming of the somatic cells with an effective amount of pluripotency factor ZSCAN10, thereby producing A-iPSCs with at least one of DNA damage response, apoptosis response, glucose metabolism, and genomic stability levels approximating those of induced pluripotent stem cells from young donors (Y-iPSCs), wherein the supplementation is carried out by adding ZSCAN10 to a culture medium in which the somatic cells are maintained or by transfecting the somatic cells with a vector harboring a nucleic acid sequence encoding ZSCAN10.