	US 11,718,830 B1
	Silicone-based membrane surface chemistry and topography control for making self-assembled cell sheets with cell alignment and positioning
Alireza Shahin-Shamsabadi, Kitchener (CA)
Filed by CaroMeats Inc., Kitchener (CA)
Filed on Aug. 8, 2022, as Appl. No. 17/882,693.
Application 17/882,693 is a continuation of application No. 17/838,284, filed on Jun. 13, 2022, abandoned.
Int. Cl. C12N 5/077 (2010.01); B33Y 10/00 (2015.01); B33Y 40/00 (2020.01); B29C 64/118 (2017.01)

CPC C12N 5/0658 (2013.01) [B33Y 10/00 (2014.12); B33Y 40/00 (2014.12); B29C 64/118 (2017.08); C12N 2500/12 (2013.01); C12N 2513/00 (2013.01); C12N 2533/20 (2013.01); C12N 2533/30 (2013.01); C12N 2533/70 (2013.01); C12N 2535/10 (2013.01); C12N 2537/00 (2013.01); C12N 2537/10 (2013.01)]

20 Claims

1. A method to create a cell sheet, the method comprising:

treating a silicone-based membrane with an aqueous solution, wherein the silicone-based membrane comprises a polydimethylsiloxane (PDMS) elastomer;

growing cells on the treated membrane;

aligning the cells on the treated membrane; and

creating a cell sheet.