US 11,718,824 B2
Synthetic attachment medium for cell culture
Stephen Joseph Caracci, Elmira, NY (US); David Henry, Fontaine le Port (FR); Jessica Jo Kelley, Keene, NY (US); Mark Alan Lewis, Horseheads, NY (US); and Yue Zhou, Horseheads, NY (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Filed by Corning Incorporated, Corning, NY (US)
Filed on May 27, 2021, as Appl. No. 17/332,461.
Application 17/332,461 is a continuation of application No. 16/371,252, filed on Apr. 1, 2019, granted, now 11,046,931.
Application 16/371,252 is a continuation of application No. 14/375,614, granted, now 10,400,210, issued on Sep. 3, 2019, previously published as PCT/US2013/024001, filed on Jan. 31, 2013.
Claims priority of provisional application 61/594,016, filed on Feb. 2, 2012.
Prior Publication US 2021/0284957 A1, Sep. 16, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); C12N 5/0735 (2010.01); C12N 5/0775 (2010.01); C12M 1/00 (2006.01); B05D 3/02 (2006.01)
CPC C12N 5/0068 (2013.01) [B05D 3/0254 (2013.01); C12M 23/20 (2013.01); C12N 5/0037 (2013.01); C12N 5/0056 (2013.01); C12N 5/0606 (2013.01); C12N 5/0663 (2013.01); C12N 2500/90 (2013.01); C12N 2500/98 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01); C12N 2533/50 (2013.01)] 20 Claims
 
1. A method for coating a surface of a cell culture article, comprising:
(i) introducing a polypeptide-polymer comprising a polymer having a covalently attached polypeptide to an aqueous cell culture medium to produce a polymer containing cell culture medium, wherein the polymer is selected from the group consisting of a polymer that has a linear backbone and is crosslink free, a polymer that has a branched backbone and that is crosslink free, and a star polymer that is crosslink free, wherein the polypeptide-polymer is soluble at 20° C. or less, wherein the aqueous solution is substantially free of organic solvents, and wherein the molar ratio of the polypeptide-polymer to polymer not covalently attached to polypeptide in the aqueous solution is from about 1:1 to about 1:50;
(ii) disposing the polymer containing cell culture medium on the surface of the cell culture article to produce a coated article; and
(iii) incubating the coated article in the medium under cell culture conditions to attach the polymer conjugated to the polypeptide to the surface of the cell culture article.