US 11,718,821 B2
Pairing probiotics and prebiotics, methods for growth and use, separately and in combination
Shayne Morris, Ogden, UT (US); and Michael Benson, Newton, UT (US)
Filed by Shayne Morris, Ogden, UT (US); and Michael Benson, Newton, UT (US)
Filed on Nov. 5, 2021, as Appl. No. 17/520,325.
Application 17/520,325 is a division of application No. 16/380,226, filed on Apr. 10, 2019, granted, now 11,168,299.
Claims priority of provisional application 62/655,935, filed on Apr. 11, 2018.
Prior Publication US 2022/0169975 A1, Jun. 2, 2022
Int. Cl. C12N 1/20 (2006.01)
CPC C12N 1/20 (2013.01) 10 Claims
 
1. A method for producing a probiotic product designed to promote and maintain health in a human, comprising:
selecting at least one probiotic organism from the group consisting of Bacteroides uniformis, Bacteroides thetaiotaomicron, Bacteroides fragilis, and Eubacteria limosum;
providing a growth broth for growing the probiotic organism, wherein the growth broth comprises 1000 mg of Glycomacropeptide, 1000 mg of Xylo-oligosaccharide, 1000 mg of Glucomannan, 1000 mg of Inulin from sprouted greens, 1000 mg of Galactomannan, 1000 mg of Blueberry powder, 1000 mg of Thymus, 1000 mg of Mulberry leaves, 1000 mg of Myo-inositol, 1000 mg of modified citrus pectin, 500 mg of Tapioca, 1000 mg of Glucosaminoglycan, 500 mg of guar gum fiber, 500 mg of oat oligosaccharide, 500 mg of Clitoria ternatea (blue pea flower), 500 mg of cranberry extract, and 500 mg of Bearberry (Arctostaphylos uva-ursi);
inoculating the growth broth with the probiotic organism;
growing the probiotic organism in the growth broth;
harvesting the probiotic organism from the growth broth;
providing an agar spread plate media for growing the probiotic organism, wherein the agar spread plate media comprises 1000 mg of Glycomacropeptide, 1000 mg of Xylo-oligosaccharide, 1000 mg of Glucomannan, 1000 mg of Inulin from sprouted greens, 1000 mg of Galactomannan, 1000 mg of Blueberry powder, 1000 mg of Thymus, 1000 mg of Mulberry leaves, 1000 mg of Myo-inositol, 1000 mg of modified citrus pectin, 500 mg of Tapioca, 1000 mg of Glucosaminoglycan, 500 mg of guar gum fiber, 500 mg of oat oligosaccharide, 500 mg of Clitoria ternatea (blue pea flower), 500 mg of cranberry extract, and 500 mg of Bearberry (Arctostaphylos uva-ursi);
inoculating the agar spread plate media with the probiotic organism harvested from the growth broth;
growing the probiotic organism on the agar spread plate media;
harvesting the probiotic organism from the agar spread plate media; and
freeze-drying the probiotic organism.