US 11,718,819 B2
Cell proliferation bioreactor
Tian Xia, Shanghai (CN); and Ke Ma, Shanghai (CN)
Assigned to Shanghai Longevity Co., Ltd., Shanghai (CN)
Filed by Shanghai Longevity Co., Ltd., Shanghai (CN)
Filed on Oct. 13, 2021, as Appl. No. 17/500,869.
Claims priority of application No. PCT/CN2021/119701 (WO), filed on Sep. 22, 2021.
Prior Publication US 2023/0090147 A1, Mar. 23, 2023
Int. Cl. C12M 1/36 (2006.01); C12M 1/26 (2006.01); C12M 1/12 (2006.01); C12M 3/00 (2006.01); C12M 1/00 (2006.01)
CPC C12M 41/48 (2013.01) [C12M 23/22 (2013.01); C12M 23/48 (2013.01); C12M 25/02 (2013.01); C12M 33/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A bioreactor comprising:
optical sensors, wherein the optical sensors are fixed and determine conditions or parameters comprising a pH, a partial pressure of dissolved oxygen (pO2), and a partial pressure of dissolved carbon dioxide (pCO2) within a culture medium vessel;
the culture medium vessel housing culture medium, the culture medium vessel further comprising:
a first side surface comprising a first transparent optical window; and
a second side surface parallel to the first side surface and comprising one or more sensor adapters to fix the optical sensors;
a cell retention vessel disposed underneath and connected to the culture medium vessel, the cell retention vessel housing biological cells and comprising:
a top surface that intersects a base of the culture medium vessel; and
a second transparent optical window indented into the top surface at a first corner of the top surface;
a semipermeable membrane disposed at a bottom of the cell retention vessel; and
a frame comprising a grid disposed underneath the semipermeable membrane;
waste collection interfaces comprising respective connectors;
valves attached to the respective connectors, wherein the valves are electronically controlled, by a processor, to automatically open and close based on the conditions or parameters; and
tubes or pipes attached to the valves through which a portion of the culture medium is expelled upon opening of the valves.