US 11,718,687 B2
Biomoletron for regulation stem cell differentiation
Jeong Woo Choi, Seoul (KR); Sang Uk Kim, Seoul (KR); Jin Ho Yoon, Seoul (KR); and Mohsen Mohammadniaei, Seoul (KR)
Assigned to SOGANGN UNIVERSITY RESEARCH FOUNDATION, Seoul (KR)
Appl. No. 16/75,790
Filed by SOGANG UNIVERSITY RESEARCH FOUNDATION, Seoul (KR)
PCT Filed Mar. 29, 2016, PCT No. PCT/KR2016/003208
§ 371(c)(1), (2) Date Aug. 6, 2018,
PCT Pub. No. WO2017/159911, PCT Pub. Date Sep. 21, 2017.
Claims priority of application No. 10-2016-0032252 (KR), filed on Mar. 17, 2016.
Prior Publication US 2019/0202939 A1, Jul. 4, 2019
Int. Cl. C12M 1/42 (2006.01); C07K 17/14 (2006.01); C07K 19/00 (2006.01); C12N 5/00 (2006.01)
CPC C07K 19/00 (2013.01) [C07K 17/14 (2013.01); C12M 35/02 (2013.01); C12N 5/00 (2013.01)] 11 Claims
 
1. A bioelectronic device for controlling stem cell differentiation, comprising:
(a) a protein comprising the amino acid sequence of SEQ ID NO: 2 having a redox potential;
(b) a first single strand DNA comprising the nucleic acid sequence of SEQ ID NO: 3 binding to the N-terminal of the protein having a redox potential;
(c) a second single strand DNA comprising the nucleic acid sequence of SEQ ID NO: 4 complimentarily hybridizing with the first single strand DNA to form a double strand DNA;
(d) a nanoparticle directly conjugated to a terminal of the second single strand DNA; and
(e) (i) a cell-penetrating peptide and (ii) a differentiation-inducing factor, which are conjugated to the nanoparticle;
wherein the cell-penetrating peptide is indirectly conjugated through a linker introduced to a surface of the nanoparticle,
wherein the differentiation-inducing factor is indirectly conjugated through a linker introduced to a surface of the nanoparticle,
wherein the differentiation-inducing factor is released from the nanoparticle by potential application,
wherein the double strand DNA further comprises metal ions between mismatch nucleotide pairs, and
wherein the number of mismatch nucleotide pairs is 1 to 10.