CPC C07K 16/18 (2013.01) [G01N 33/502 (2013.01); G01N 33/53 (2013.01); G01N 33/563 (2013.01); G01N 33/564 (2013.01); G01N 33/577 (2013.01); G01N 33/6857 (2013.01); G01N 2496/80 (2013.01); G01N 2800/285 (2013.01)] | 12 Claims |
1. A method of diagnosing multiple sclerosis (MS) in a human patient comprising the steps of
(a) incubating a sample from the human patient selected from the group consisting of blood, serum and plasma with a DNA-replication-associated (Rep) protein comprising,
(i) the amino acid sequence as depicted in SEQ ID NOS: 1 or 14; or
(ii) a fragment of the amino acid sequence as depicted in SEQ ID NO: 1 and the fragment consists of the amino acid selected from the group consisting of the amino acids 1 to 136 or 137 to 229 of SEQ ID NO:1 and SEQ ID NOS: 2, 3 or 9;
(b) detecting the amount of antibodies in the sample from the human patient forming an immunological complex with the Rep protein;
(c) determining the extent of difference in the amount of antibodies present in the sample from the human patient and a control sample taken from a healthy individual who has not been diagnosed for MS; and
(d) diagnosing MS, when the amount of antibodies bound to Rep protein in the sample from the human patient is at least 2-fold increased, as compared to the amount in the control sample.
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