CPC C07K 14/4737 (2013.01) [A61K 47/42 (2013.01); C07K 14/4726 (2013.01); G01N 33/569 (2013.01); G01N 33/56911 (2013.01); G01N 33/56916 (2013.01); G01N 33/56938 (2013.01); G01N 33/56944 (2013.01); G01N 33/6854 (2013.01); A61K 38/00 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01); G01N 2333/4737 (2013.01)] | 16 Claims |
1. A method for determining the presence or absence of a microbe in a sample, comprising:
(i) contacting a test sample with a microbe-targeting molecule; and
(ii) detecting binding of a microbe to the microbe-targeting molecule,
wherein the microbe is present in the test sample if binding is detected and wherein the microbe-targeting molecule comprises:
a. at least one first domain comprising at least a portion of a c-reactive protein (CRP);
b. at least one second domain comprising at least a portion of a domain selected from the group consisting of:
(i) Fc region of an immunoglobulin;
(ii) microbe-binding domain of a microbe-binding protein, wherein the microbe-binding protein is not CRP;
(iii) neck region of a lectin;
(iv) a detectable label;
(v) domain for conjugation to surface of a carrier scaffold;
(vi) pattern recognition receptor domain of CRP; and
(vii) any combinations of (i)-(vi); and
c. a linker conjugating the first and second domains, and
wherein the microbe-targeting molecule is a multimeric molecule.
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