US 11,718,651 B2
CRP capture/detection of gram positive bacteria
Alexander L. Watters, North Andover, MA (US); Donald E. Ingber, Boston, MA (US); Mark J. Cartwright, West Newton, MA (US); Michael Super, Lexington, MA (US); Martin Rottman, St. Cloud (FR); Evangelia Murray, Worcester, MA (US); and Brendon Dusel, West Roxbury, MA (US)
Assigned to PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Filed by PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Filed on May 12, 2021, as Appl. No. 17/318,343.
Application 17/318,343 is a division of application No. 16/669,805, filed on Oct. 31, 2019, granted, now 11,034,744.
Application 16/669,805 is a division of application No. 15/105,298, granted, now 10,513,546, issued on Dec. 24, 2019, previously published as PCT/US2014/071293, filed on Dec. 18, 2014.
Claims priority of provisional application 61/917,705, filed on Dec. 18, 2013.
Prior Publication US 2021/0277079 A1, Sep. 9, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/47 (2006.01); G01N 33/68 (2006.01); G01N 33/569 (2006.01); A61K 47/42 (2017.01); A61K 38/00 (2006.01)
CPC C07K 14/4737 (2013.01) [A61K 47/42 (2013.01); C07K 14/4726 (2013.01); G01N 33/569 (2013.01); G01N 33/56911 (2013.01); G01N 33/56916 (2013.01); G01N 33/56938 (2013.01); G01N 33/56944 (2013.01); G01N 33/6854 (2013.01); A61K 38/00 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01); G01N 2333/4737 (2013.01)] 16 Claims
 
1. A method for determining the presence or absence of a microbe in a sample, comprising:
(i) contacting a test sample with a microbe-targeting molecule; and
(ii) detecting binding of a microbe to the microbe-targeting molecule,
wherein the microbe is present in the test sample if binding is detected and wherein the microbe-targeting molecule comprises:
a. at least one first domain comprising at least a portion of a c-reactive protein (CRP);
b. at least one second domain comprising at least a portion of a domain selected from the group consisting of:
(i) Fc region of an immunoglobulin;
(ii) microbe-binding domain of a microbe-binding protein, wherein the microbe-binding protein is not CRP;
(iii) neck region of a lectin;
(iv) a detectable label;
(v) domain for conjugation to surface of a carrier scaffold;
(vi) pattern recognition receptor domain of CRP; and
(vii) any combinations of (i)-(vi); and
c. a linker conjugating the first and second domains, and
wherein the microbe-targeting molecule is a multimeric molecule.