US 11,718,644 B2
Purified protein compositions and methods of production
Aniket Kale, Pleasanton, CA (US); and Ranjan Patnaik, South San Francsico, CA (US)
Assigned to CLARA FOODS CO., South San Francisco, CA (US)
Filed by Clara Foods Co., South San Francisco, CA (US)
Filed on Oct. 27, 2022, as Appl. No. 18/50,213.
Application 18/050,213 is a continuation of application No. PCT/US2022/038074, filed on Jul. 22, 2022.
Claims priority of provisional application 63/225,410, filed on Jul. 23, 2021.
Claims priority of provisional application 63/225,388, filed on Jul. 23, 2021.
Prior Publication US 2023/0073271 A1, Mar. 9, 2023
Int. Cl. C07K 1/36 (2006.01); B01D 15/36 (2006.01); C07K 1/16 (2006.01); B01D 61/16 (2006.01)
CPC C07K 1/36 (2013.01) [B01D 15/362 (2013.01); B01D 15/363 (2013.01); B01D 61/16 (2013.01); C07K 1/16 (2013.01); B01D 2311/04 (2013.01); B01D 2311/2697 (2022.08)] 30 Claims
OG exemplary drawing
 
1. A method for preparing a protein product having a reduced quantity of a plurality of recombinant cell byproducts, the method comprising steps of:
(1) obtaining a composition comprising a recombinant protein and a plurality of recombinant cell byproducts comprising at least one human-detectable flavor or odor selected from the group consisting of an acid, an alcohol, an aldehyde, an aromatic, an ester, and a ketone;
(2) processing the composition under conditions that separate the recombinant protein from the plurality of recombinant cell byproducts, thereby obtaining a separated recombinant protein,
wherein the processing step comprises contacting the composition with an anionic resin that reversibly attaches to the recombinant protein and does not substantially attach to the plurality of recombinant cell byproducts; and
(3) collecting the separated recombinant protein, thereby obtaining a protein product having a reduced quantity of the plurality of recombinant cell byproducts;
wherein the composition comprising the recombinant protein and the plurality of recombinant cell byproducts is a cell culturing medium which contained recombinant cells that secreted the recombinant protein and contained the plurality of recombinant cell byproducts.